A VALIDATED HPTLC METHOD FOR DETERMINATION OF TERBINAFINE HYDROCHLORIDE IN PHARMACEUTICAL SOLID DOSAGE FORMAbstract
An HPTLC method for estimation of Terbinafine hydrochloride in its tablet formulation has been developed. It employs aluminium backed silica gel 60 F254 TLC plates,( 100×50 mm, thickness of layer 0.2 mm), prewashed with methanol and dried at room temperature)and mobile phase comprising of Acetonitrile: 1, 4 dioxan: Hexane: Acetic acid (1:1:8:0.1) (v/v/v/v). The developing solvent was run upto 70 mm in Camag chamber. Densitometic scanning was then performed with Camag TLC scanner-3 at λmax 282 nm. The Rf value was found to be 0.45. The linearity and range for Terbinafine Hydrochloride was found to be 500-4500ng/spot. The method was validated for precision, accuracy and sensitivity.
K.K. Patel * and V.V. Karkhanis
Department of Quality Assurance, C.U. Shah College of Pharmacy and Research, Wadhvan, Surendranagar, Gujarat, India
10 July, 2012
22 August, 2012
28 October, 2012