A VALIDATED RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF AMLODIPINE AND LISINOPRIL IN PHARMACEUTICAL DOSAGE FORM
AbstractA reverse phase high performance liquid chromatography (RP-HPLC) method for the simultaneous estimation of amlodipine and lisinopril in marketed formulation is developed. The determination was carried out on a Phenomenex C18 (250 x 4.6 mm, 5 µm) column using a mobile phase of 0.02 M phosphate buffer solution: methanol (75:25v/v, pH 7.0). The flow rate was 1.0ml/min with detection at 212 nm. The retention time for amlodipine was 4.11 min and for lisiopril 7.29 min. Amlodipine and Lisinopril showed a linear response in the concentration range of 10-110 µg/ml. The correlation co-efficient (‘ r ‘ value) for amlodipine and lisinopril was 0.9991 and 0.9992, respectively. The results of analysis have been validated statistically and by recovery studies. The percentage recoveries obtained for amlodipine and lisinopril ranges from 100.04 to 100.57%.
Article Information
17
1712-1715
409
1447
English
Ijpsr
Vijendra Chauhan*, Shailesh T. Prajapati and Chhaganbhai N. Patel
Deptartment of Quality Assurance, Shri Sarvajanik Pharmacy College, Near Arvind Baug, Mehsana- 384001, Gujarat, India
29 March, 2011
28 April, 2011
27 June, 2011
http://dx.doi.org/10.13040/IJPSR.0975-8232.2(7).1712-15
01 July, 2011
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