A VALIDATED RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF ASPIRIN AND PRASUGREL IN TABLET DOSAGE FORMAbstract
Aim of the present work is to develop a rapid, simple, precise, accurate and reproducible reverse phase high performance liquid chromatographic method for simultaneous determination of Aspirin and Prasugrel in tablet dosage form. The estimation was carried out on a HIBAR (Lichrospher C-18) column with the dimensions of 250mm x 4.6mm, 5µm. Combination of Acetonitrile and 0.5% Potassium dihydrogen phosphate buffer (adjusted to pH-3 using orthophosphoric acid) in the ratio of 60: 40 was used as mobile phase. The flow rate is set at 1.0ml/min and eluents were monitored at 220 nm. Both drugs were properly resolved having run time of 3.3 min and 4.8 min for Aspirin and Prasugrel, respectively. The method was validated as a final verification of method development with respect to Precision, Linearity, Accuracy, Ruggedness and Robustness. Linearity for Aspirin and Prasugrel was in the range of 10-450μg/ml and 10-500μg/ml respectively. The mean recoveries obtained for Aspirin and Prasugrel were within the range of 98-102%.
P. Vivek Sagar *, T. Samidha , M. Vamshi Krishna and S. Shobha Rani
Care College of Pharmacy Oglapur (v), Atmakur (M), Warangal, Telangana, India
22 April, 2014
23 July, 2014
31 July, 2014
01 November, 2014