A VALIDATED RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF METRONIDAZOLE AND CIPROFLOXACIN HYDROCHLORIDE IN PHARMACEUTICAL DOSAGE FORM
AbstractA reversed-phase-liquid chromatographic (RP-HPLC) method was developed for the determination of Metronidazole (MTZ) and Ciprofloxacin HCl (CPX) in their bulk drug and marketed formulations. The separation was carried out using a mobile phase of 25mM Potassium dihydrgen phosphate buffer of pH 3.0 consisting of 0.15%v/v Triethylamine, 0.1% Phosphoric acid and Methanol in the ratio of( 60:40v/v). The column used was Zorbax C18 column (250mm x 4.6mm, 5 µm) with flow rate of 1 ml / min using UVD 17 Detector. Detection carried out at 320 nm. The retention times of Metronidazole and Ciprofloxacin Hydrochloride were found to be 3.7 min and 5.6 min, respectively. Developed methods were validated according to ICH guidelines. Linearity was observed at concentration range of 50-250 μg/ml for Metronidazole and 62.5-312.5μg/ml for Ciprofloxacin Hydrochloride followed by Beer’s law. The percentage RSD for the method precision was found to be less than 2%. The proposed method is precise, accurate, selective and rapid for simultaneous determination of Metronidazole and Ciprofloxacin Hydrochloride.
Article Information
37
4736-4740
372KB
1795
English
IJPSR
S.S. Khadabadi * and Meenakshi G. Devkar
Department of Pharmaceutical Chemistry, Government college of Pharmacy, Osmanpura, Aurangabad-431005, Maharashtra, India
khadabadi@yahoo.com
17 July, 2013
27 August, 2013; accepted, 19 November, 2013; published 01 December, 2013 A VALIDATED RP-HPLC METHOD
19 November, 2013
http://dx.doi.org/10.13040/IJPSR.0975-8232.4(12).4736-40
01 December 2013
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