A VALIDATED RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF METRONIDAZOLE AND CIPROFLOXACIN HYDROCHLORIDE IN PHARMACEUTICAL DOSAGE FORMAbstract
A reversed-phase-liquid chromatographic (RP-HPLC) method was developed for the determination of Metronidazole (MTZ) and Ciprofloxacin HCl (CPX) in their bulk drug and marketed formulations. The separation was carried out using a mobile phase of 25mM Potassium dihydrgen phosphate buffer of pH 3.0 consisting of 0.15%v/v Triethylamine, 0.1% Phosphoric acid and Methanol in the ratio of( 60:40v/v). The column used was Zorbax C18 column (250mm x 4.6mm, 5 µm) with flow rate of 1 ml / min using UVD 17 Detector. Detection carried out at 320 nm. The retention times of Metronidazole and Ciprofloxacin Hydrochloride were found to be 3.7 min and 5.6 min, respectively. Developed methods were validated according to ICH guidelines. Linearity was observed at concentration range of 50-250 μg/ml for Metronidazole and 62.5-312.5μg/ml for Ciprofloxacin Hydrochloride followed by Beer’s law. The percentage RSD for the method precision was found to be less than 2%. The proposed method is precise, accurate, selective and rapid for simultaneous determination of Metronidazole and Ciprofloxacin Hydrochloride.
S.S. Khadabadi * and Meenakshi G. Devkar
Department of Pharmaceutical Chemistry, Government college of Pharmacy, Osmanpura, Aurangabad-431005, Maharashtra, India
17 July, 2013
27 August, 2013; accepted, 19 November, 2013; published 01 December, 2013 A VALIDATED RP-HPLC METHOD
19 November, 2013
01 December 2013