A VALIDATED RP-HPLC METHOD FOR THE ESTIMATION OF ANAGRELIDE IN CAPSULE DOSAGE FORMSAbstract
An accurate and precise high performance liquid chromatographic method was developed for quantitative estimation of anagrelide in capsule dosage forms. Chromatographic separation of the drug was achieved on a Kromosil C18 column (150 x 4.6 mm; 5µ) by isocratic elution using a mobile phase consisting of phosphate buffer (pH 2.5) and acetonitrile (75:25 v/v) at a flow rate of 1.2 mL/min. The drug in the eluate was monitored by UV detection at 250 nm. Under optimized conditions, the retention time obtained for the drug was 4.818 min. The calibration curve was linear over the range of 50-150 µg/mL of the drug. The recovery of drug by the proposed method was found to be 98.95% to 100.45%. The performance of the method was validated as per ICH guidelines. The method was also found to be applicable for determination of anagrelide in its capsule dosage forms without any interference from the excipients. The proposed method is suitable for routine quality control analysis of anagrelide.
K. Sujatha*, C. Palavan , J.V.L.N. Seshagiri Rao
Senior Lecturer, Department of Pharmacy, Government Polytechnic, Visakhapatnam – 530 007, Andhra Pradesh, India
13 May, 2013
17 June, 2013
15 September, 2013
01 October, 2013