A VALIDATED STABILITY-INDICATING HPTLC-PDA METHOD FOR TOLNAFTATE BULK AND STANDARD DRUG

To estimate Tolnaftate in the presence of its forced declination products, a straightforward and quick stability indicating High Performance Thin Liquid Chromatography -Photo Diode Array (HPTLC-PDA) approach was created and validated. The methodology used Toulene: Chloroform: Methanol (311 v/v) as mobile phase and SunQSil C18 column (250mm×4.6mm, 5µm) as stationary phase. Retention time of tolnaftate was found to be 0.27 minutes. To assess the declination conduct of tolnaftate, examinations into acid, alkali, oxidative, UV declination and thermal declination were conducted. In agreement with the criteria of International Conferences on Adjustment (ICH), the created and optimized approach was validated. The results showed that the limits of quantitation and discovery were 0.421 and 0.088µg/mL, singly. With R2=0.9991, linearity was seen in the attention range of 0.276-6µg/mL. Results showed the recovery ranged from 98.2 to 101.01. Reversed 7 Phase -High Performance Thin Liquid Chromatography (RP-HPTLC), a developed and tested technology was used to quantify tolnaftate in an in-house topical result. The main products of Oxidative and Base declination were discovered and described using electrospray ionization mass spectrometry and liquid chromatography.