A VALIDATED STABILITY INDICATING RP – HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF METFORMIN AND TENELIGLIPTIN IN BULK AND PHARMACEUTICAL DOSAGE FORMAbstract
This is a simple, economic, sensitive stability indicating RP-HPLC method for the simultaneous estimation of metformin and teneligliptin in bulk and pharmaceutical formulation. The method was carried out on octa-decyl C18 column (5 μm, 25 cm × 4.6 mm, i.d) using methanol: water in the ratio of 70:30 and pH of the mobile phase up to 3 was adjusted with OPA at a flow rate of 1.0 ml/min. The wavelength for metformin and teneligliptin at 235 nm was found to be appropriate. The linearity range was obtained in the concentration range of 25 – 150 μg/ml for MET, while 5 – 30 μg/ml for TNG respectively. The retention time of metformin and teneligliptin was found to be 2.45 and 6.68 min, respectively. The regression equation for MET and TNG were found to be as y = 8.288 x + 0.026 and y = 27.26 x + 38.28 with correlation coefficient (R2) 0.999 and 0.999, respectively. The developed method was found to be robust, accurate and sensitive which can be used for estimation of combination of metformin and teneligliptin in pharmaceutical dosage forms.
A. R. Shaikh *, B. A. R. K. Ahmed and M. Ibrahim
M.C.E. Society’s Allana College of Pharmacy, Azam Campus, Camp, Pune, Maharashtra, India.
05 July, 2017
17 September, 2017
20 October, 2017
01 April, 2018