ACTIVITY OF ETHYL ACETATE FRACTIONS OF TONGKAT ALI ROOTS (EURYCOMA LONGIFOLIA, JACK) ON BLOOD GLUCOSE LEVELS IN STREPTOZOTOCIN INDUCED DIABETIC RATS
AbstractThis study is to evaluate two ethyl acetate fractions of “Tongkat Ali Roots” (TAR) namely fractions-1 and fraction-2 (F-1; F-2) on blood glucose levels in streptozotocin (STZ) induced diabetic rats. Each fraction used sixty rats which grouped into six groups (n=10). Group 1 is normal rats which received aqua dest; group 2 is diabetic rats which received STZ 60 mg/kg b.w., group 3 is diabetic rats with standard anti-diabetic metformin 10 mg/kg b.w. and group 4, 5, and 6 are diabetic rats which received tested fractions at doses of 25, 50 and 100 mg/kg b.w. Diabetic rats were induced by STZ intraperitoneally. The fasting blood glucose were assessed on days 0, 7th after diabetic inducing and 15th after giving tested fraction by using a digital glucometer (GCU Easy Touch) from the tip of the tail vein rat. The data were analyzed by using one-way analysis of variance (ANOVA). The fraction-2 (F-2) which was made from the extract of TAR by Vacuum Liquid Chromatography (VLC) using mobile phase ethyl acetate-ethanol-water in ratio 80: 30: 1 has potency as anti-diabetic (P<0.05) when compared with fraction 1 using the same mobile phase in ratio 100: 30: 1. This study demonstrated the fraction-2 has the ability to lowering blood glucose. The fifty effective dose (ED50) values of F-2 are 1.71 mg/dL and 67.48 mg/dL for F-1 on days 15th after giving tested fractions.
Article Information
25
2290-2293
547
1248
English
IJPSR
H. Yusuf *, K. Hasballah and K. F. Jamil
Department of Pharmacology, Faculty of Medicine, University of Syiah Kuala, Indonesia.
hans_yusuf1104@yahoo.com
17 August 2018
24 November 2018
06 December 2018
10.13040/IJPSR.0975-8232.10(5).2290-93
01 May 2019