ACUTE AND SUBACUTE TOXICITY STUDIES OF LIVAMURTHA, TRADITIONAL FORMULATION

Livamurtha, traditional polyherbal formulation possess hepato-protective, antibacterial, and antihepatotoxic properties used for treating liver disorders. This exploratory study was designed to evaluate the acute and sub acute toxicity effects of traditional formulation in albino mice based on the OECD Guidelines. 10g of formulation ”Livamurtha” was boiled in 500ml of water for 1 hour. The decocation (Filtrate) obtained was evaporated and later were reconstituted in water for further analysis. In acute toxicity study, healthy albino mice were chosen for study with starting single dose of 5mg/kg/body weight followed by single doses of 50,300 and 2000mg/Kg/body weight in separate group of each 3 animals. The animals were observed for 14 days and its physical activities were noted. In sub toxicity study, the aqueous extract of formulation was administered orally at repeated dose of 300 and 600 mg/kg body weight for 28 days. The animals were sacrificed on the 29^th day for further haematological, biochemical and histopathological examinations were done. In acute toxicity, no behavioural changes, mortality and toxicity was observed in mice during 14 day observational period.In sub acute toxicity study, there was no significant observational changes in the experimental animals. There is no evidence of toxicity as exhibited by regular observations, body weight, food consumption etc., No significant biochemical and histopathological changes were observed at the end of the study. Livamurtha, traditional formulation proves to be nontoxic and the therapeutic effect of it can be reached by the synergetic activity of phytoconstituents present in the formulation pharmacodynamically.