ALPHA-AMYLASE INHIBITORY ACTIVITY OF KATANKATERYADI KWATHA (K. KWATHA) IN MANAGEMENT OF TYPE 2 DIABETES

Objectives: The objective of the study is to find out the alpha-amylase inhibitory activity of Katankateryadi Kwatha. The alpha-amylase enzyme is the key source for postprandial hyperglycaemia, which is responsible for Type 2 Diabetes. Methodology: The crude drugs of Katankateryadi Kwatha (K. Kwatha) were collected from a local market. The Kwatha was prepared and dried with rotatory evaporator, under reduced pressure. Different concentrations (10, 20, 40, 60, 80 and 100 μg/ml.) of Kwatha were prepared and used for α-amylase inhibitory assay using starch as a substrate. Similarly, the protocol performed with Acarbose as standard. The absorbance was read at 595 nm using spectrophotometer. The percentage of α-amylase inhibitory activity and IC₅₀ values of each concentration was calculated. Results & Discussion: The study shows that the Katankateryadi Kwatha has alpha-amylase enzyme inhibitory property. Standard Acarbose shows IC₅₀ value of 38.46±0.956 with % inhibition ranged from 23% to 80% at concentration 10, 20, 40, 60, 80 and 100 µg/ml. while the inhibitory concentration (IC₅₀ value) of K. Kwatha was found to be 68.69±1.0890 with % inhibition on alpha-amylase ranged from 9% to 64% at concentration 10, 20, 40, 60, 80, and 100 µg/ml. Conclusion: The evidence show that K. Kwatha (a natural polyherbal product) has alpha-amylase inhibitory activity. The results of K. Kwatha are satisfactory and highly significant in comparison to standard Acarbose. Therefore K. Kwatha a polyherbal decoction, might be a future potent Ayurvedic formulation for diabetic patients.