AN RP-HPLC METHOD FOR THE QUANTITATIVE DETERMINATION OF REMDESIVIR IN INTRAVENOUS DOSAGE FORM
AbstractThe worldwide epidemic of Coronavirus disease 2019 (COVID-19), caused by a new virus known as severe acute respiratory syndrome (SARS) coronavirus 2, has posed a growing threat to public health (SARS-CoV-2). The only antiviral drug authorized by the FDA for treating adult and pediatric patients hospitalized with a severe disease is remdesivir, which is given intravenously (IV). Although only a few methods for estimating remdesivir in pharmaceutical formulations using high-pressure liquid chromatography (HPLC) have been described, its determination still requires an accurate, precise, quick, and easy analytical methodology. The main goal of this study was to develop and validate a reliable and accurate HPLC method for quantitative estimation of remdesivir in its intravenous dosage formulation. The separation was performed on a C18 (4.6 mm x 150 mm, 5.0 µm) column with a flow rate of 0.7 mL/min and a total run duration of 6 minutes using a simple isocratic mobile phase of acetonitrile and 0.1 percent formic acid. The method was validated for the system suitability, linearity, precision, accuracy, robustness, and others as per the International Council for Harmonization (ICH) Q2 (R1) guideline. The results show that the method for measuring remdesivir using HPLC is simple, quick, sensitive, accurate, precise and robust. The described approach was successfully used to quantify remdesivir in a commercially available pharmaceutical formulation.
Article Information
24
1273-1279
615 KB
468
English
IJPSR
Kashyap Thummar *, Malay Pandya, Nisarg Patel and Foram Rawat
Graduate School of Pharmacy, Gujarat Technological University, Ahmedabad, Gujarat, India.
kasu_patel5@yahoo.co.in
02 July 2022
21 August 2022
01 September 2022
10.13040/IJPSR.0975-8232.14(3).1273-79
01 March 2023