AN UPDATED REVIEW ON MOLECULAR GENETICS, PHYTOCHEMISTRY, PHARMACOLOGY AND PHYSIOLOGY OF BLACK NIGHTSHADE (SOLANUM NIGRUM)HTML Full Text
AN UPDATED REVIEW ON MOLECULAR GENETICS, PHYTOCHEMISTRY, PHARMACOLOGY AND PHYSIOLOGY OF BLACK NIGHTSHADE (SOLANUM NIGRUM)
Ashwani Kumar*, S. Sagwal, Niketa and S. Rani
Department of Bioscience, Shri Ram College Muzaffarnagar, Uttar Pradesh, India
This article reviews, connected the gap between the folkloric use of Solanum nigrum and the results of evidence based experiments. Although Solanum nigrum is a rich source of one of plants most dreaded toxins solanine, it has appreciably monstrated its potential as a reservoir of antioxidants having hepatoprotective, anti-tumor, cytostatic, anti-convulsant, anti-ulcerogenic and anti-inflammatory.effects. The review encompasses in-vitro, in vivo and clinical studies done on Solanum nigrum, while examining.whether or not correct scientific measures have been taken in generating experimental evidences for its traditional uses. This review would afford research scientist to know how much is known and what is left undone in the investigation of Solanum nigrum. The compounds considered in this revieware flavonoids and other phenolics, alkaloids (especially indole terpenoid and purine alkaloids), essential oils and other terpenoids, cannabinoids, lucosinolates and isothiocyanates, and compounds having human hormone activity. The review concludes with a discussion of the possible evolutionary mechanisms that have led to the evolution of UV-B regulation of secondary metabolite accumulation. Many Ayurveda philosophers and healers praised about the properties of this plant and utilized in various disorders. Here, a review made on the screening of Solanum nigrum for various activities. It is found that the drug is very potential and can be used for various applications as mentioned in Ayurveda. Black nightshade grows as a weed, found in the dry parts of India and other parts of the world. It has a medicinal usage and has been used as a traditional folk medicine for treating various ailments such as pain, fever and liver disorders. Generally, black nightshade is very rich in nutritive values, which are capable of supplying minerals, vitamins, hormones and proteins. This herb elaborates a wide variety of medicinal properties such as anticancer, antioxidant, neuroprotective, cytoprotective, antimicrobial, antinociceptive and antipyretic properties.
INTRODUCTION: Nature has provided a complete store-house of remedies to cure all aliments of mankind. This is where, nature provides us drugs in the form of herbs, plant and algae’s to cure the incurable diseases without any toxic effect 1. Solanum nigrum European Black nightshade or locally just (black nightshade). A species in the Solanum genus, native to Eurasia & introduced in the America, Australasia & South Africa 2. The Solanum nigrum complex also known as solanum L is group of Solanum species with general black nightshade characteristic. The Solanum species in this group can be taxonomically confused, more-so by intermediate forms and hybridization between the species 1.
Solanum incanum, Solanum nigrum (Solanaceae)in the plant family Solanaceae (night shadow plant) the genus solanum is very large group of about 1400 species found throughout in the temperate and tropical region of the world like Solanum aviculare (Europe, New Zealand), S. dulcamara (Europe), S. incanum (Africa), S. khasianum (India subcosmopolite), S.laciniatum (New Zealand,Australia), S.nigrum (cosmopolite), S.pseudocapsicum(an ornamental, cultivated in greenhouses), S. tuberosum (potato) and S. melongena (eggplant, aubergine) 3.
Black nightshade is a fairly common herb or short-lived perennial surb found in many wooded areas as well as disturbed habitats. It has a hight of 30-120 cm, leaves 4-7 cm and 2-5 cm wide long with a winged upper portion. The flowers have petals greenish to whitish, recovered when aged and surround prominent bright yello anthers. The berry is mostly 6-8 mm diameter dull black or purple-black in India 4. The toxicity of Solanum nigrum varies widely depending on the variety and poisonous plant experts advise” unless you are certain that the berries are from an edible strain leave them alone 5. All parts of plant can be poisonous, containg toxic glycoalkaloids at 0.524% (dry weight) 6.
The toxins are most concentrated in the unripe green berrie 7, 6. Poisoning symptoms are typically delayed for 6 to 12 hours after ingestion 7. The solanine in Solanum nigrum is not destroyed during normal cooking because the decomposition temperature of solanine is about 243 C 8.
Several compounds have been isolated from different fractions of S.nigrum which have shown pharmacological relevance to the observed effects of whole plant preparation of S.nigrum. Acetic acid, tartaric acid, malic acid and citric acid were identified as the major organic acids in S.nigrum 9. Tartaric acid and citric acid however, were said to be most important in adaptive responses by S. nigrum to environmental stresses.
High concentrations of solanine, a glycoalkaloid is found in most parts of Sn, but highest levels are found in unripe berries of S. nigrum. However, when ripe, the berries are the least toxic part of the plant and are sometimes eaten without ill effects. Similarly, the solanine increases in the leaves as the plant matures. The absolute amount of alkaloid per leaf increased during leaf development, whereas, the concentration declined. Small unripe fruits of S. nigrum had a high concentration of solasodine, but both the concentration and the absolute amount per fruit decreases with fruit maturation 10.
Studies on S. nigrum through spectroscopic analysis, chemical degradation and derivitisation led to the identification of six new steroidal saponins collectively called solanigrosides and a one known saponin degalactotigonin 11. The effect of crude polysaccharide isolated from S. nigrum linn. (SNL-P) was examined both in vivo and in vitro on U14 cervical cancer cells 12. This can also be considered as the basis for its use as an anticancer agent 13.
Consequently, the need for potent antioxidants in our diet and drug supplements becomes very necessary. A study which utilises six pretreatment methods before cooking on the peroxidase activity, chlorophyll and antioxidant status of S. nigrum L, sharp difference in the carotenoids, phenolics, flavonoids and tannins contents has been reported, indicating the fragility of this antioxidant present in S. nigrum 14. This is an herbal plant that has been used as hepatoprotective and anti-inflammatory agent in Chinese medicine 15. Central nervous system-depressant action of S. nigrum was ascertained by measuring the effects of intraperitoneal injection of S. nigrum on various neuropharmacological parameters 16.
Their biosynthesis has also been implicated in the pathophysiology of cardiovascular diseases, cancer, colonic adenomas and Alzheimer’s 17.
In Ethiopia, the ripe berries are picked and eaten by children in normal times, while during famines all affected people would eat berries; it is used as a food source until their crops are ready 18. The Welayta people in the nearby Wolayita Zone do not weed out S. nigrum that appear in their gardens since they likewise cook and eat the leaves 19.
In South Africa, the very ripe and hand-selected fruit (nastergal in Afrikaans and umsobo in Zulu) is cooked into a beautiful but quite runny purple jam 20. The plant has a long history of medicinal usage, dating back to ancient Greece. It was a traditional European medicine used as a strong sudorific, analgesic and sedative with powerful narcotic properties 21. Internal use has fallen out of favor in Western herbalism due to its variable chemistry and toxicity, but it is used topically as a treatment forherpes 22.
S. nigrum is an important ingredient in traditional Indian medicines. Infusions are used in dysentery, stomach complaints and fever. The juice of the plant is used on ulcers and other skin diseases. The fruits are used as a tonic, laxative, appetite stimulant; and also for treating asthma and "excessive thirst" 23.Traditionally the plant was used to cure tuberculosis24.
In North India, the boiled extracts of leaves and berries are also used to alleviate liver-related ailments, including jaundice. In Assam, the juice from its roots is used against asthma and whooping cough 25. S.nigrum is a widely used plant in oriental medicine. It is antitumorigenic, antioxidant, anti-inﬂammatory, hepatoprotective, diuretic, and antipyretic 26.
Plant Molecular biology, tissue culture and physiology of Solanum nigrum: Solanum nigrum found in different forms and different metheod used by many scincetist in this process. The psbA gene coding for this protein was cloned from Solanum nigrum atrazine-susceptible ('S') and atrazine-resistant ('R') biotypes. Non-silent change in psbA in different 'S' and 'R' weed biotype pairs suggests a functional, herbicide-related role for this codon position 27. The psbA gene cloned in pSB135 from Solanum nigrum atrazine-resistant biotype was sequenced 28.
Another study results demonstrate the existence of a marked antagonism between physicochemical stresses, with water stress enhancing the resistance of photosystem II to constraints (heat, strong light at high temperature) that are usually associated with drought in the field 29. The open reading frame and terminator region of a wound-inducible tomato Inhibitor I gene, regulated by the CaMV 35S promoter, was stably integrated into the genomes of nightshade (Solanum nigrum), and two other plantsusing an Agrobacterium-mediated transformation system. The results of these experiments suggest that maximal expression of foreign proteinase inhibitor genes, and perhaps other foreign defense genes, may require gene constructs that are fashioned with promoters and terminators that allow maximum expression in the selected plant species 30.
A wound-inducible proteinase Inhibitor I gene from tomato containing 725 bp of the 5' region and 2.5 kbp of the 3' region was stably incorporated into the genome of black nightshade plants (Solanum nigrum) using an Agrobacterium Ti plasmid-derived vector. The results demonstrate that thegene contains elements that can be regulated in a wound-inducible, tissue-specific manner in nightshade plants31.
Some scincetist study based on Suspension cell cultures of Solanum nigrum were grown in the presence of six different chloroplast DNA synthesis inhibitors. These results suggest that DNA gyrases participate in cpDNA replication 32. A number of Solanum nigrum mutants resistant to the antibiotics spectinomycin, streptomycin and lincomycin have been isolated from regenerating leaf stripsafter mutagenesis with nitroso-methylurea.The value of these mutants for studies on plastid genetics is discussed 33.
Efficient plastid transformation has been achieved in Nicotiana tabacum using cloned plastid DNA of Solanum nigrum carrying mutations conferring spectinomycin and streptomycin resistance. It is a diminished mismatch, recombination/repair system in higher-plant plastid34. In protoplast-derived Solanum nigrum microcalluses, plasmodesmal connectivity and cell division behaviour of the sister cells were examined by repeated pressure-injection experiments with the fluorescent dye Lucifer Yellow (LYCH; M (r) 457) and concomitant light-microscopical long-term live observations.
The results are discussed with respect to the possible role of plasmodesmata in exerting "supracellular control" over mitotic activity by trafficking mitosis-regulating signals 35. Reactive oxygen species (ROS) production, peroxidase activity and lipid peroxidation have been studied in the CF-treated cell suspensions derived from leaves of the resistant S. Nigrum (nonhost) and S. tuberosum cv. Bzura as well as from the susceptible S. Tuberosum cv. They suggest that lack of stringent control of the oxidative processes and sensitivity to the pathogen toxins may be decisive for limited polygenic resistance in potato 36.
Many scientist studies were based on Solanaceae family plant S.nigrum and otherplant species. They were demonstrated that transgenic Lotus plants producing opines (which are small amino acid and sugar conjugates) specifically favour growth of opine-degrading rhizobacteria 37. They suggest that Piper extracts could be used effectively as contact botanical insect control agents to protect potato plants from developing L. decemlineata larvae at concentrations less than 0.1%. There is also potential for Piper extracts to control insecticide resistant populations in conjunction with other integrated pest management (IPM) strategies used in conventional and organic agriculture 38.
In breeding for resistance to late blight, (Phytophthora infestans Mont. De Bary), an economically important disease affecting potatoes, the search for new sources of durable resistance includes the non-host wild Solanum species. The results confirm the effective transfer of late blight resistance of S. nigrum into its somatic hybrids with potato 39. Previous studies have shown that suspension-cultured cells of Solanum genotypes with various polygenic resistances to Phytophthora infestans differed in activities of early oxidative processes in response to culture filtrate (CF) from this pathogen. The relative increase in the ROS production was higher in the susceptible clone H-8105 than in both resistant genotypes. Lipid peroxidation increased only in the nonhost S. nigrum.
The present results suggest that the involvement of both ROS production and LOX activity in the defense strategy in Solanum species/P. infestans interactions 40. Tomato plastid transformants were obtained using two vectors containing cloned plastid DNA of either Nicotiana tabacum or Solanum nigrum and including point mutations conferring resistance to spectinomycin and streptomycin. Transformants were recovered after PEG-mediated direct DNA uptake into protoplasts, the results demonstrate the efficacy in tomato of a selection strategy which avoids the integration of a dominant bacterial antibiotic resistance gene 41.
Some scientist of the study based on different plant species for different techniques used in this process. Based on the photosynthesis-light response equation, the theoretic light compensation point of L. serriola was 37.58 micromole m (-2) x s(-1), its theoretic light saturation point was 1 rate of L. serriola were leaf photosynthetic active radiation,stomatal 42.
They extend Ryan's seminal work on the 18-amino acid polypeptide systemin in tomato's (Solanum lycopersicum) systemic wound response to the closely related solanaceous species Solanum nigrum 43. The influence of the acetolactate synthase inhibitor metsulfuron-methyl on the operation of the photosynthetic apparatus was examined on 4-weeks-old climate chamber-grown Solanum nigrum plant. To have an indication on the relative performance of the photosynthetic apparatus of ALS-treated plants Results indicated a progressive inhibition of the level of CO(2) fixation, the relative quantum efficiency of photosystem I (Phi(PSI)) and II (Phi(PSII)) electron transport and the leaf chlorophyll content already 2 days after application of the herbicide 44.
This study based on the technology of obtaining the tobacco plants possessing the hereditary changes in the photosynthesis pigments accumulation during development using exogenous DNA has been elaborated (Solanum nigrum L.) These plants have simultaneously the useful features--accelerated development, early blooming phenotype and higher productivity. Possible mechanisms emphasized such inherited biochemical changes have been discussed 45.
The team of scincetist study on the analysis of the structure and functional relationship was carried out by docking of the beta-(1, 3)-glucan onto the acidic cleft region on the surface of the protein (SniOLP) 46. The initial phase of the lipid peroxidation process in leaves of Solanum nigrum var. gigantea, Solanum tuberosum CV Bzura and clone H-8105, which represent non-host resistance, field resistance and susceptibility, respectively, against Phytophthora infestans, was investigated. The obtained results are discussed in light of the overall biochemical cell status of plants in the studied interactions 47. Heterologous protein expression in microorganisms may contribute to identify and demonstrate antifungal activity of novel proteins.
The Solanum nigrum osmotin-like protein (SnOLP) gene encodes a member of pathogenesis-related (PR) proteins; it represents a novel PR-5 protein with promising utility for biotechnological applications 48. The present study is intended to determine metabolites of 12 dichlorinated, seven trichlorinated, five tetrachlorinated and one entachlorinated PCB congener transformed by black nightshade (Solanum nigrum) hairy root culture SNC-9O. Only traces of metabolites were found in sporadic cases, so exudation of unbound biphenylols from the cells is not expected 49.
Many scincetist study based on S.nigrum plant species.Gibberellins (GAs) are endogenous hormones that play a predominant role in regulating plant stature by increasing cell division and elongation in stem internodes. The product of the GA 2-oxidase gene from Phaseolus coccineus (PcGA2ox1) inactivates C (19)-GAs. The PcGA2ox1 gene was introduced into Solanum melanocerasum and S. Nigrum (Solanaceae) by Agrobacterium-mediated transformation with the aim of decreasing the amounts of bioactive GA in these plants and thereby reducing their stature 50.
Various in vitro grown tissues (non-regenerative callus, regenerative callus and microshoot derived leaves) of Solanum nigrum L. were cultured under salinity stress (0-150 mM NaCl) for enhanced production of solasodine, a steroidal alkaloid and an alternative to diosgeninThe proposed HPTLC method showed a good linear relationship (r2=0.994) in 50-2000ng/spot concentration ranges. The data demonstrate that the solasodine production in cultures was growth dependent 51. By using genetic transformation of Agrobacterium rhizogenes and liquid culture, The results showed that hairy roots could be initiated from the cut edges of leaf explants 5 days after inoculation with the strain of A. rhizogenes ATCC15834. The results presented here had provided the possibilities on how to prepare optimum medium for large scale cultivation and production of solasodine from S. nigrum L. var. pauciflorum hairy roots 52.
Solanaceaeous taxa produce diverse peptide serine proteinase inhibitors (SPIs), known antidigestive defenses that might also control endogenous plant proteases. While the defense against herbivores specialized on SPI-rich diets requires other unknown defense mechanisms 53. Fertilizer amendments can impact weed populations in a variety of ways. Compost is a promising tool for incorporation into integrated weed control strategies aimed at reducing weed seed bank persistence 54.
This study based on naturally selected atrazine-resistant (AR) weeds possessing a Ser(264) --> Gly D1protein en coded by a mutant psbA allele in the chloroplast-DNA have increased photosensitivity and lower fitness. Suggesting that the evolutionarily conserved D1 structure must be indispensable for the efficient NPQ process in higher plants 55. The tomato red spider mite Tetranychus evansi Baker ET Pritchard occurs on solanaceous plants, and causes serious damage to a variety of crops in Africa and Europe. In 2001 this species was also found in Japan, on nightshade (Solanum nigrum L.). These results indicate that T. evansi after invasion into Japan has the potential to become a serious pest on solanaceous crops, just the same as in Africa and Europe 56.
Remediation of plant-microorganism-chelates synergy has been proposed as an effective remediation method for enhancing the removal efficiency of heavy metal. The responses of antioxidative enzymes to Cd stress significantly decreased following application of CA and PLNH1, and the oxidative stress experienced by the plant due to Cd in the soil 57. Premise of the study: Intron Targeting (IT) primers were developed for potato using expressed sequence tags (EST) and NCBI database records to study genetic diversity. Twenty-nine polymorphic intron targeting (IT) markers were generated and characterized from 30 samples of potato and 22 samples of Solanum nigrum to detect polymorphism. The developed markers will provide valuable tools for genetic diversity analysis, genetic mapping, and marker-assisted breeding of potato and related Solanum species 58.
Zn tissue accumulation in Solanum nigrum grown in a non-contaminated and a naturally contaminated Zn matrix and the effect of inoculation with different arbuscular mycorrhizal fungi (AMF) on metal uptake were assessed 59. This study was carried out to determine the fungal pathogens on S.nigrum L. and other six species is involved in this process. As a result of two year surveys, ten fungal pathogens were determined on eight weed species.
Further studies should be conducted to evaluate the efficacy of this pathogen under in vitro and in vivo conditions 60. Jasmonates are ubiquitous messengers in land plants essential for the activation of defense responses. Solanum nigrum plants transformed to silence the expression of key genes in jasmonate production (SnLOX3) 61.
This study based on Black nightshades is a group of species best known for their 'poisonous' or noxious weedy reputation. This high level of novel genomic variability obviously enabled species to succeed in their new environment 62. Arbuscular mycorrhizal fungi (AMF) are known for their beneficial effects on plants. Our results show that AMF can negatively influence the growth of some weed species indicating that AMF have the potential to act as determinants of weed community structure, in order to further the practical and ecological relevance of our findings, additional experiments should be performed under field conditios 63.
Gibberellins (GAs) control many aspects of plant development, including seed germination, shoot growth, flower induction and growth and fruit expansion. The results are discussed in the context of regulating plant stature, since this strategy would decrease the use of chemicals to promote plant growth 64.
Ecological and environmental studies: Ecological and environmental studies in different plant species is used, accumulation and endurance of 45 weed species in 16 families the results showed that Solanum nigrum and Conyza canadensis can not only accumulate high concentration of Cd, but also strongly endure to single Cd and Cd-Pb-Cu-Zn combined pollution. Although there were high Cd-accumulation in Artemigia selengensis, Znula britannica and Cephalanoplos setosum, their biomass was adversely affected due to action of heavy metals in the soils 65.
Plants respond to environmental stresses through a series of complicated phenotypic responses, which can be understood only with field studies because other organisms must be recruited for their function. Present Solanum nigrum L. a Solanaceous relative of potato and tomato for which many genomic tools are being developed, as a model plant ecological expression system. Both flea beetle attack and jasmonate elicitation increased proteinase inhibitors and jasmonate elicitation decreased fitness in field-grown plants. Hence, proteinase inhibitors and jasmonate-signalling are targets for manipulative studies 66.
The aim of our investigations was to study the susceptibility of 22 weed species to Pepino mosaic virus (PepMV). Seven plants of each species were mechanically inoculated at 4-6 leaf stage with PepMV in a vector free virological glasshouse. Inoculated plants were tested on the basis of symptoms, by DAS ELISA serological method and back inoculation. Among the 22 weed species, Other 18 weed species seemed to be resistant to PepMV, and on the basis of back inoculation not even latent infection has been observed 67.
This study investigated the allelopathic effects of various weeds extracts on seed germination of 11 crop species. Most of the weed extracts tested had inhibitory effects on seed germination of common bean, tomato, pepper, squash, onion, barley, wheat, and corn at different application rates as compared with the 10% acetone control. It was concluded that some of the weed extracts tested in this study could be used as inhibitor while others could be used as stimulator for the crops 68.
It is main groundwork and the first step of phyto-extraction of its commercial application on a large scale to screen out a series of ideal hyperaccumulators that can effectively remedy contaminated soil by heavy metals, A cadmium-hyperaccumulator S. nigrum L. (weed) was first discovered by using the pot-culture method arranged in outdoor and sampling-analyzing experiments carried out in heavy metal contaminated areas 69.
The influence of different cadmium concentrations on the organic acid level in leaves of the Cd hyperaccumulator, S. nigrum L. in particular the relationship of organic acids with Cd accumulation in S.nigrum was investigated based on the pot-culture experiment. These results indicated that tartaric, acetic and citric acids in leaves of S. nigrum might act as the indication of Cd hyperaccumulation 70. S. nigrum was found to proliferate in sediments with high levels of metal pollution.
The effect of Zn on plant growth and tissue metal accumulation was assessed. The response of the plant to the inoculation with four different isolates of arbuscular mycorrhizal fungi (AMF) (Glomus sp. BEG140, Glomus claroideum, Glomus mosseae and Glomus intraradices) was studied 71.
This study based on allelopathic effect of Ageratum conyzoides L. Cynodon dactylon (L.)Pers. Parthenium hysterophorus L. and Solanum nigrum L. were examined on seed germination, seedling growth, total protein content and protein profile on Ankur, Bhatt, Bragg, PK -416, PS-1042 and Shilajeet varieties of soybean (Glycine max (L) Merill). The order of susceptibility of different varieties with different weed extracts followed the order: Ankur > PK-416 > Bhatt > Shilajeet > Bragg and > PS-1042 72.
The plant species composition and their ability to accumulate heavy metals were investigated at three contaminated sites in Xiangxi area, Southern China. The concentrations of Cd, Pb, Zn, and Cu in more than 363 samples of 125 plant species were analyzed in the present study. Such as Kalimeris indice (L.) Sch.-Bip. and Solanum nigrum L. might be suitable for use in the phytoextraction of contaminated soils.
The dominant and relative dominant species with low accumulation of metals and dense fibrous root systems, such as Imperata cylindrical (L.) Beauv. Var. major C. E. and Miscanthus floridulus (Labill.)Warb. Might be suitable for stabilizing such metal contaminated sites 73.
The behaviour of the organochlorine pesticide hexachlorocyclohexane (HCH) is investigated. The concentrations of alpha-, beta-, gamma-, and delta-HCH isomers were measured in soils, rhizosphere and vegetation in a contaminated area in Galicia (NW Spain). The total concentration of HCH in soils reached values close to 20,000 mgkg-1.
The plants analysed (Avena sativa L. Chenopodium spp. Solanum nigrum L. Cytisus striatus (Hill) Roth and Vicia sativa L.) accumulated HCH, especially the beta-HCH isomer; in their tissues tend to reduce levels of the HCH isomers in the rhizosphere. The results reflect the importance of vegetation in the distribution of organochlorine compounds in thesoil-plant system 74.
This study based on six new steroidal saponins, solanigrosides C-H (2-7), and one known saponin, degalactotigonin, were isolated from the whole plant of Solanum nigrum. Their chemical structures were elucidated using spectroscopic analysis, chemical degradation, and derivatization. All seven compounds were tested for their cytotoxicity using four human tumor cell lines (HepG2, NCI-H460, MCF-7, and SF-268). Only compound 1 was cytotoxic, with IC50 values of 0.25-4.49 microM 75.
Desmedipham, phenmedipham and a 50% mixture of the two decreased the maximum quantum efficiency of photosystem II (F (v)/F (m)) and the relative changes at the J step (F (VJ)) immediately after spraying in both sugar beet and black nightshade grown in the greenhouse. The differential speed of herbicide metabolism between weed and crop plays an important role in herbicide selectivity and can be studied by using appropriate chlorophyll a fluorescence parameters 76.
Topramezone is a new, highly selective herbicide of pyrazole structure for the post-emergence control of broadleaf and grass weeds in corn. The biokinetic properties and mode of action of topramezone were investigated in plants of Setaria faberi Herrm, Sorghum bicolor (L.)Moench, Solanum nigrum L. and the crop species corn (Zea mays L.). Within 2-5 days after treatment, topramezone caused strong photo-bleaching effects on the shoot, followed by plant death of sensitive weeds. A more rapid metabolism combined with a lower sensitivity of the 4-HPPD target enzyme contributes to the olerance of corn to topramezone 77.
Hyperaccumulators are ideal plant species used for phytoremediation of soils contaminated by heavy metals. A full understanding of metal tolerance mechanisms of hyperaccumulators will facilitate enhancing their phytoremediation efficiency when exposed to a higher Cd level (48 mg kg-1), growth and most N metabolism indicators were reduced significantly 78. The effect of two different chelating agents [EDTA and EDDS (S, S-ethylene diamine-dissucinic acid)] on Zn tissue accumulation in Solanum nigrum L. grown in a naturally contaminated soil was assessed.
Under those conditions, addition of chelating agents did not seem to have an effect on the localisation of accumulation sites. The devise of a chelate-enhanced phytoextraction strategy, using chelating agents and AMF, is discussed 79.
In this study based on the phytotoxic and antimicrobial properties of olive mill wastes have been widely investigated and demonstrated over the past decade. The study shows the high potential of naturally occurring chemicals present in TPOMW and TPOMW composts that should be further investigated as bio-pesticides for their use in sustainable agricultural systems 80. Zn accumulation in Solanum nigrum grown in naturally contaminated soil in the presence of different types of organic amendments was assessed. S. nigrum grown in the non-amended soil always presented higher Zn accumulation in the tissues 81.
After simulated herbivory, inflicted by a treatment involving wounding and the application of Manduca sexta oral secretions (OS) to mechanical wounds (OS-elicitation), transcripts of the systemin-precursor, prosystemin, are down-regulated in black nightshade (Solanum nigrum). That down-regulation of systemin after herbivory is associated with increased root allocation which allows plants to more effectively compete with conspecifics and may allow plants to compensate for tissue losses during herbivory 82.
Application of synthetic chelates such as ethylene diamine tetraacetic acid (EDTA) has been proposed as an alternative technology for phytoextraction of contaminated soils. In a pot experiment, the effects of EDTA application at three growing stages on growth and Cd uptake and accumulation of Solanum nigrum L. Were investigated. The results indicated that moderate rate of EDTA applied at the flowering stage would be important to enhance phytoremediation efficiency in practice 83.
This study based on Solanum nigrum is a newly discovered Cd-hyperaccumulator. In the present study, the protective effects of proline against cadmium toxicity of callus and regenerated shoots of S. nigrum are investigated based on a high frequency in vitro shoot regeneration system.
The interaction between proline and enzymic or non-enzymic antioxidants is discussed 84. Study on dye-sensitized solar cells (DSSCs) with extracts of Canna indica L. Salvia splendens, Solanum nigrum L. as sensitizer is firstly reported in this paper. DSSCs were assembled by using natural dyes extracted from C. indica L., S. splendens, cowberry and S. nigrum L. as sensitizers. The electrochemical impedance spectroscopy (EIS) was used to analyze the interface resistance of cells. The result indicated that high resistance 85.
Some scincetist studied that phytoremediation is a promising tool in removing pollutants from the environment or in rendering them harmless. Results showed that iron-deficiency induced cadmium uptake, biomass decrease and changes in pH and Eh in hydroponic culture. Bioconcentration and translocation factors indicated that iron-deficiency status affected cadmium accumulation and translocation in Solanum nigrum L. 86.
Cadmium concentrations in two plant species and their corresponding soils were evaluated in a metal contaminated area. The average Cd concentrations reached 36.9 and 141 mg kg-1 in Solanum nigrum leaves and Lobelia chinensis shoots, respectively. Under the hydroponic culture conditions, the maximum Cd concentration in the S. nigrum leaves and L. chinensis shoots were 1,110 and 414 mg kg (-1), respectively. Cd concentration was higher in the roots than in the aerial parts. The two plants may be used in suitable phytoremediation process 87.
Polybrominated diphenyl ethers (PBDEs) are used as additive flame retardants PBDEs are persistent, bioaccumulative and toxic compounds. They are often detected in sewage sludge which is applied on agricultural soils as fertilizer. To our knowledge this is one of the first studies reporting the accumulation of both lower PBDEs and BDE 209 in plants. Our results suggest that absorption, accumulation and trans-location of PBDEs by plants and their transfer to the food chain could represent another possible risk for human exposure 88. The elevated Cd accumulation in plants in response to salt was found to be correlated with the elevated levels of phytochelatin the expression of heavy metal transporters AtHMA1-4, especially AtHMA4.
Salt alleviated growth inhibition caused by Cd and increased Cd accumulation also was observed in Cd accumulator Solanum nigrum 89. This study based on S.nigrum L. a novel technology to obtain highly efficient biosorbent from the endophytes of a hyperaccumulator is reported. Carboxyl, amino, sulphonate and hydroxyl groups on EF LSE10 surface were responsible for the biosorption of cadmium. Copyright (c) 2009 Elsevier Ltd. All rights reserved 90.
To study if Solanum nigrum hairy roots can be used for phytoremediation of Cd contamination, we investigated the effects of cadmium (Cd) alone, and in combination with different concentrations of CaCl2, on growth, activities of superoxide dismutase (SOD) and peroxidase (POD) and Cd absorption by hairy roots of S. nigrum L. var pauciflorum. The results showed that Cd concentrations of lower than 50 micromole/L enhanced the growth of hairy roots, while higher than 100 micromol/L inhibited growth and decreased the number of branched roots. This was achieved on one hand by reducing the absorption of Cd, on the other hand by decreasing the lipid peroxidation through regulating the activities of antioxidant enzymes SOD and POD in the hairy roots 91.
The effects of cadmium (Cd) on the accumulation of hydrogen peroxide H2O2 and antioxidant enzyme. Activities in roots of Solanum nigrum L. and the role of N-acetyl-l-cysteine (NAC) as a cysteine (Cys) donor against Cd toxicity were investigated. These results suggest that NAC could protect plants from oxidative stress damage, and this protection seems to be performed via increased GSH biosynthesis. Furthermore, NAC treatment also increased the contents of protein thiols in S. nigrum roots 92.
Phytoremediation is a cost-effective, simple and sustainable beneficiary technique to purify the polluted environment. Solanum nigrum L. a newly found cadmium (Cd) hyperaccumulator has shown the potential to remediate Cd-contaminated soils. Present study investigated the effects of fertilizer amendments on the Cd uptake by S. nigrum. Chicken manure and chicken manure may be a better fertilizer for phytostabilization.2009 Elsevier B.V. All rights reserved 93. A radiation dose assessment exercise was carried out for the edible biota Solanum nigrum, Carica papaya, Raphnus sativum and Phaseolus domesticus due to naturally available radionuclides (40) K, (238) U and (232) in the Domiasiat area in Meghalaya, India. Finally compared with the IAEA and UNSCEAR data set for screening level dose risk assessment 94. Valuable endophytic strains facilitating plants growth and detoxification of heavy metals are required because the application of plant-endophyte symbiotic system is a promising potential technique to improve efficiency of phytoremediation.
In this study, endophytic bacterium LRE07 was isolated from cadmium hyperaccumulator Solanum nigrum L. indicating that the endophyte possesses specific and remarkable heavy metal remediation abilities 95. A well-characterized cadmium (Cd) hyperaccumulating plant Solanum nigrum was grown in Cd and polycyclic aromatic hydrocarbons (PAHs) co-contaminated soil that was repeatedly amended with chemicals, including EDTA, cysteine (CY), salicylic acid (Sa), and Tween 80 (TW80), to test individual and combined treatment effects on phytoremediation of Cd-PAHs contaminated soils.These results indicated that S. nigrum might be effective in phytoextracting Cd and enhancing the biodegradation of PAHs in the co-contaminated soils with assistant chemicals 96.
In this study, Solanum nigrum was used in-situ for Cd phytoremediation in Cd polluted soil on Shenyang Zhangshi Irrigation area (SZIA) in 2008. The results in this paper provide reference values for the future research on the application of Solanum nigrum L. in phytoremediation and on chemical or/and agricultural strategies for phytoextraction efficiency enhancement 97. This study investigates the heavy metal-resistant bacterial endophytes of Cd-hyperaccumulator Solanum nigrum L. grown on a mine tailing by using cultivation-dependent technique.
Thirty Cd-tolerant bacterial endophytes were isolated from roots, stems, and leaves of S. nigrum L. and classified by amplified ribosomal DNA-restriction analysis into 18 different types 98. This study based on S.nigrum in jasmonate signalling plays a central role in activating the plethora of responses that are elicited by herbivory. Solanum nigrum plants silenced in the expression of genes involved in jasmonic acid biosynthesis (irlox3), conjugation (irjar4) and perception (ircoi1) were used to study the function of these genes in the field and in the regulation of transcriptional and metabolic responses 99. The effects of Bacillus sp. SLS18, a plant-growth-promoting endophyte, on the biomass production and Mn/Cd uptake of sweet sorghum (Sorghum bicolor L.), Phytolacca acinosa Roxb. And Solanum nigrum L. were investigated. SLS18 displayed multiple heavy metals and antibiotics resistances. This is not only gives a promising phytoremediation strategy but also eases the competition for limited fertile farmland between energy crops and food crops 100.
Pharmacological and Ethanopharmacological aspects: S. nigrum and other plant species is involved in this study in a recent survey, 106 local healers in Israel were interviewed concerning the use of Solanaceae as medicinal plants. The main findings reveal that the extensive distribution of modern, safe narcotics, sedatives and anaesthetics has reduced the use of the Solanaceae for these purposes 101. The ethanol extract of the fruit of Solanum nigrum L. (Solanaceae) was studied for its neuropharmacological properties on experimental animals. The observations suggest that the fruit of S. nigrum possesses potential CNS-depressant action 102.
The preparations of Solanum nigrum L. leaves were made on Biomphalaria alexandrina. Extract (A), made by soaking leaves powder over night in cold 70% ethanol, has the highest activity, (LC50 3.37 mg/L within 24 hr). This extract also showed larvicidal activity against larvae of two mosquito species, Aedes caspius and Culex pipiens, (LC50 51.29 and 125.89 mg/L within 24 hr, and 21.38 and 38.11 mg/L within 48 hr, espectively). Sunlight, pH, and turbidity did not affect the activity of this extract, but diluted solutions of this extract lost their activity after four weeks 103.
Allelopathic effect of four plant specieswere examined on seedling growth of certain commonly used varieties of wheat (Triticum aestivum L.) in the Tarai region of U.P. state. The weed extracts inhibited the length of plumule in all the varieties (100%) with Solanum and it was in 12 (92%), 10 (77%) and 06 (46%) varieties with Polygonum, Avena and Cyperus, respectively. On the basis of the present results, UP--2003 and WH--542 followed by PBW--226, Sangam and HD--248 were more susceptible to all the four weed extracts compared to the rest of the varieties of wheat 104.
Nightshade berries containing glycoalkaloids can be a contaminant in green peas. Methodology was developed to detect this contamination. None of the samples contained alpha-solasonine. No unripe berries of Solanum nigrum were detected visually in the samples 105.
The present study based on ethnomedicine survey covers the Dharwad district of Karnataka in southern India. It was revealed that 35 plants belonging to 26 families are being used to treat different types of oral ailments like toothache, plaque and caries, pyorrhea and aphthae. Sixteen of these plants were new claims for the treatment of oral ailments not previously reported in the ethnomedicinal literature of India and Solanum nigrum are used to treat tooth ache are used in the treatment of plaque and caries 106.
Two main research questions are framing this investigation: the main taxa of the medicinal importance value altered the Showbak forest stand and species composition? The most safe species and what are the toxic ones (unsafe). Obtaining results is relied on the interviewee's personal information and the medicinal use of specific plants 107. Studies using the medicinal plants enumerated in this study, particularly those with high number of citations and high F (IC) values might yield some novel prototypes. Such studies will also be useful to assess the efficacy and safety of these herbal treatments to take decisions on the health care of rural India 108.
Phytochemical and biochemical studies: Phytochemical studies was performed on bioactivity-guided fractionation, three known steroidal glycosides, beta 2-solamargine, solamargine, and degalactoti-gonin, were isolated from Solanum nigrum. The structures of 1-3 were elucidated on the basis of chemical evidence and spectral analysis, especially by 2D-NMR analysis 109.
Two new steroidal saponins, named nigrumnins I and II, together with two known saponins were obtained from the whole plant of Solanum nigrum L. On the basis of spectroscopic analysis (1H-NMR, 13C-NMR, 1H-1H COSY, TOCSY, HMQC, HMBC and FAB-MS), nigrumnin I was established as (25R)-5alpha-spirostan-3beta-ol 3-O-betaD-xylopyranosyl-(1-->3)-[alpha-L-arabinopyranosyl-(1 -->2)]-beta-D- glucopyranosyl-(1-->4)-[alpha-L-rhamnopyranosyl(1-->2)]-beta-D-galacto pyranoside (1), and nigrumnin II was elucidated as (25R)-3beta,17alpha-dihydroxy-5alpha-spirostan-1 2-one, 3-O-beta-D-xylopyranosyl-(1-->3)-[alpha-L-arabino pyranosyl-(1-->2)]-beta-D-glucopyranosyl-(1-->4)-[alpha-L-rhamnopyra-nosyl-(1-->2)l-beta-D-galacto pyranoside (2) 110.
The importance of the glycolytic flux for the success of Biomphalaria-Schistosome sporocyst interaction was acertained in this study. Hexokinase (HK), pyruvate kinase (PK. Effect of LC25 of Solanum nigrum leaves dry powder as plant molluscicide on HK, PK and GPI were tested. Treatment with this plant resulted in a significant inhibition of these three investigated enzymes. LC10 concentrations of S. nigrum reduced considerably the infection rate of B. alexandrina with S. mansoni to be 34% compared to an infection rate of 80% in control, non-treated snails 111.
A rapid, easy, and simple spectrophotometric method was developed for the estimation of total alkaloids precipitated by Dragendorff's reagent (DR) in plant materials. It is based on the formation of yellow bismuth complex in nitric acid medium with thiourea. Using this method, in eight plant species is used in this process the alkaloidal percentage of certain alkaloids itcan be used for routine analysis of commercial samples by industries dealing with herbal drugs for standardization of plant materials containing alkaloids and for alkaloid-containing pharmaceutical products 112.
Various physiological imbalances lead to reactive oxygen species (ROS) overproduction and/or increases in lipoxygenase (LOX) activities, both events ending in lipid peroxidation of polyunsaturated fatty acids (PUFAs). Besides the quantification of such a process, the development of tools is necessary in order to allow the identification of the primary cause of its development and localization ROS-mediated peroxidation was mainly driven by light and always appeared as a late process 113.
The study on the absorption and accumulation of heavy metals lead, zinc, copper and cadmium by 8 plant species around a smelter showed that the metals accumulation by plants differed with plant species, their parts, and kinds of metals.
Abutilon theophrasti had a higher capability of absorbing and accumulating Pb. These plants had TF values higher than 1, and were suitable for phytoextraction to remedy polluted soil 114.
Two new pregnane saponins, solanigroside A (1) and solanigroside B (2), along with two known compounds (3 and 4), were isolated from 60% ethanolic extract of the dried herb of Solanum nigrum L. on the basis of extensive spectroscopic analysis as well as comparison with reported spectroscopic data of related compounds. This paper deals with the isolation and structural characterisation of pregnane glycosides from S. nigrum L. 115.
The separation was performed on the silica gel CC, Sephadex-LH20 CC as well as preparative HPLC. The constituents were isolated and identified by spectral methods. Five compounds isolated from 60% ethanol extract were identified as 6-methoy-hydroxycoumarin (I), syringaresinol-4-O-beta-D-glucopyranoside (II), pinoresinol-4-O-beta-D-glucopyranoside (III), 3, 4-dihydroxhbenzoic acid (IV), p-hydroxybenzoic acid (V), 3-methoxy-4-hydroxyienzoic acid (VI), adenosine (VII) II, III, and VII were isolated from this genus for the first time 116.
In this study carried out investigation some scientist study based on wild vegetables play an important role in the diet of inhabitants of different parts of the world. Among the wild vegetables of South Africa are Chenopodium album, Sonchus asper, Solanum nigrum and Urtica urens. All the vegetables had comparatively lower concentrations of phytate, alkaloids and saponins 117.
Being able to rapidly and sensitively detect specific enzymatic products is important when screening biological samples for enzymatic activity. The fluorescence and MALDI-TOF-MS signal decrease were associated with the inhibitory effect of the PIs on trypsin. The developed platform can be modified to screen novel protease inhibitors, namely, those potentially useful for treating or preventing infection by viruses, including HIV and hepatitis C 118. A gene encoding a preprohydroxyproline-rich systemin, SnpreproHypSys, was identified from the leaves of black nightshade (Solanum nigrum) and some many other plant species that structural conformations within the peptides are recognized by the different cells/species to initiate signal transduction pathways, apparently through recognition by homologous receptor(s). To further demonstrate the biological relevance of the SnHypSys peptides, the early defense gene lipoxygenase D was shown to be induced by all three synthetic peptides when supplied to excised nightshade plant 119.
Phytochemical analysis of Solanum nigrum has resulted in the isolation of two novel disaccharides. Their structures were determined as ethyl beta-D-thevetopyranosyl-(1-->4)-beta-D-oleandropyranoside (1) and ethyl beta-d-thevetopyranosyl-(1-->4)-alpha-D-leandropyranoside (2), respectively, by chemical and spectroscopic methods 120.
This study based on Solanum nigrum L. (SN) has exhibited multiple biological effects such as anti-inflammation and antiproliferation. Protein kinase C (PKC) regulates cellular functions including proliferation, migration, and invasion. In the present investigation, our results revealed the antimigration and anti-invasion effects of both extracts derived from SN, which may act as a promising therapeutic agent for the treatment of hepatocellular carcinoma 121. To study the chemical constituents from Solanum nigrum.Compounds were isolated and purified by silica gel, Sephadex LH-20 and preparative HPLC. Their structures were identified by physicochemical properties and spectral analysis.
Six compounds were isolated and identified as (+)-pinoresinol (I), (+)-syringaresinol (II), (+)-medioresinol (III), scopoletin (IV), tetracosanoic acid (V) and beta-sitosterol (VI). Compounds I - III are isolated from this genus for the first time, while compounds IV and V are isolated from this plant for the first time 122. S. nigrum Linn. (Solanaceae), a traditional Chinese medicine (TCM), has been used for cancer therapy.
It is urgent to develop a novel quality standard to validly detect its quality. To control its quality, a novel, accurate and valid fingerprint method was developed by high-performance liquid chromatography-evaporative light scattering detection (HPLC-ELSD) in the current case. So, in order to get the consistent raw materials, the collecting location and the harvesting time should be fixed 123.
A new method for simultaneous determination of solasonine (1), solamargine (2) and khasianine (3) in Solanum nigrum by reversed-phase HPLC was developed. The samples were separated at 30 degrees C on Agilent Zorbax SB C18 (4.6 mm x 150 mm, 5 microm) column with acetonitrile-water-phosphoric as mobile phase. The method is rapid, simple and accurate, and it can be used for the evaluation of S.nigrum L. 124. A new characteristic steroidal glycoside possessing a hydroxyl group at C-23, inunigroside a (1), was isolated from the withered berries of S.nigrum L. A new bisdesmoside, 3-O-β-D: -glucopyranosyl-(1→4)-β-D: -glucopyranosyl-(1→4)-β-D: -glucopyranosyl (22R, 25S)-3β, 15α-dihydroxyspirost-5-ene 15-O-α-L: -rhamnopyranoside (4), named jasminoside A, was isolated from the methanolic extract of S. jasminoides 125.
Pharmacological and medicinal activities: The team of scientist study based on pharmacological & medicinal activities in this process based on S.nigrum and other plant activities .I this study18 plant species, Solanum nigrum, was evaluated and discussed. The sensitivity of the keratinophilic fungi was evaluated by dry-weight method 126. Prosystemin is the precursor protein of the 18 amino acid wound signal systemin which activates systemic defense in tomato leaves against insect herbivores (McGurl B, Pearce G, Orozco-Cardenas M, Ryan CA, Science 255 (1992) 1570-1573).
Here, we report the isolation of cDNA sequences encoding prosystemin from potato (Solanum tuberosum), black nightshade (S. nigrum), and bell pepper (Capsicum annuum), all members of the Solanaceae family, using reverse-transcription polymerase chain reaction (RT-PCR). The accumulation of proteinase inhibitor mRNA transcripts could be induced in each of these plants by treatment with the homologous systemin. As in the tomato, in potato, black nightshade, and bell pepper plants, prosystemin homologs appear to function as precursors of systemic wound signals 127.
Ultrastructural effects of AAL-toxin TA from Alternaria alternata on black nightshade (Solanum, nigrum L.) leaf discs and correlation with biochemical measures of toxicity. In black nightshade (Solanum nigrum L.) leaf discs floating in solutions of AAL-toxin TA (0.01-200 microM) under continuous light at 25oC, electrolyte leakage, chlorophyll loss, autolysis, and photobleaching were observed within 24 h. Electrolyte leakage, measured by the conductivity increase in the culture medium 128. This study based on Solanum nigrum L. leaves and fruits were shown to have molluscicidal activities against snails transmitting schistosomiasis and fascioliasis. Toxicity of plant extracts was also affected by other seasonal dependent factors. These are the duration of plant exposure to direct sunlight and the size of the fruits. S. nigrum (black fruits) was more toxic (LC50 = 18.1) than the other two types, S. nigrum, V. vellosum (yellow fruits) (LC50 = 38.9) and S. nigrum v. juidaicum (red fruits) (LC50 = 34.7) 129.
Squamous cell carcinoma of the esophagus is endemic in parts of South Africa. Previous case-control studies have shown many associations but no clear etiologic pathway has been established. Solanum nigrum, beans, and pumpkin all contain protease inhibitors. Suppression of protease inhibitors can lead to overexpression of growth factors in the esophagus, resulting in a proliferative and oncogenic drive 130. The molluscicidal properties of Solanum nigrum L. were tested against three Egyptian snail species (Biomphalaria alexandrina, Bulinus truncatus and Lymnaea natalensis), each an intermediate host of parasites causing human schistosomiasis or fascioliasis.
The concentrations needed to kill all cercariae (LC100) within 30 min of exposure were 30 mg/litre for both S. haematobium and S. mansoni and 40 mg/litre for F. Gigantica 131. The presence of plant extracts of Solanum nigrum and Cichorium intybus in the reaction mixture containing calf thymus DNA and free radical generating system protect DNA against oxidative damage to its deoxyribose sugar moiety. These studies suggest that the observed hepatoprotective effect of these crude plant extracts may be due to their ability to suppress the oxidative degradation of DNA in the tissue debris 132.
A study of 100 patients with cancer of the esophagus and 100 controls matched for sex, age, and educational level was done in Transkei, with extensive inquiries into diet and social habits. The significant risk factors found were use of Solanum nigrum as a food (relative risk, 3.6), smoking (relative risk, 2.6), and use of traditional medicines (relative risk, 2.1).
Consumption of traditional beer was not a risk factor 133. Wistar albino female rats were maintained for 10 d on diets containing various levels of the vegetable Solanum nigrum. Simultaneously, they received daily intraperitoneal injections of aflatoxin B1 (AFB1) (either 0.2 or 0.4 mg/kg body-weight) diluted in propylene glycol. Similar results were obtained with glutathione S-transferase (EC 18.104.22.168) activity which increased by 60% with diet S600 134.
Antiulcerogenic activities of three plant drugs were studied against aspirin-induced gastric ulcers in rats. The activity may be due to inhibition of acid and pepsin secretions and their in vitro ability to bind these. Brassica oleracea (leaf) powder did not affect the ulcer index significantly but its aqueous extract lowered the index. This suggests that its antiulcerogenic effect is due to decreases of acid and pepsin outputs which enhance gastric mucosal strength. The reference drug gefarnate decreased the ulcer index by increasing the hexosamine level only.cimetidine inhibited the acid production but did not decrease the ulcer index 135.
A group of scientist studies based on phytoecdysteroids (agonists) are widely distributed in the plant world, but solanaceous species have not been extensively examined for their presence. We have now surveyed 128 species of solanaceous plants for the presence of ecdysteroid agonist and antagonist activities using the Drosophila melanogaster B (II) cell line bioassay. Each of the phytoecdysteroid-accumulating species examined (Browallia speciosa, Nierembergia hippomanica var violacea, N. solanacea and Solanum nigrum) contain a cocktail of ecdysteroids, of which 20-hydroxyecdysone and polypodine B (5beta, 20-dihydroxyecdysone) are major components 136.
The 50% ethanol extract of the whole plant of Solanum nigrum was tested in vitro for its cytoprotection against gentamicin-induced toxicity on Vero cells. Cytotoxicity was significantly inhibited as assessed by the Trypan blue exclusion assay and mitochondrial dehydrogenase activity (MTT) assay. The test extract also exhibited significant hydroxyl radical scavenging potential, thus suggesting its probable mechanism of cytoprotection 137. Some reschers on studied the susceptibility or resistance of Solanum capsicastrum Link. Et Schauer, S. comatum Sendt, S. dulcamara L, S. luteum Mill, S. Malacoxylon Sendt. and S. nigrum L. to three aphid transmissible viruses [alfalfa mosaic alfamovirus (AMV), potato M. carlavirus (PVM) and potato S carlavirus (PVS)]. Out of the species, S. capsicastrum, S. comatum, S. dulcamara, S. malacoxylon and S. nigrum to AMV, S. capsicastrum and S. malacoxylon to PVM and S. capsicastrum, S. luteum and S. nigrum to PVS showed the highest resistance (immunity). Solanum comatum and S. dulcamara were susceptible to PVS. Symptoms (necrotic lesions, mosaic and chlorosis) could be seen after inoculation and the absorbance values (DAS-ELISA) exceeded twice that of the healthy control samples during the serological tests 138.
The plant Solanum nigrum treated with the pathogen Phytophthora infestans-derived elicitor responded by elevated reactive oxygen species (ROS) production, lipid peroxidation and lipoxygenase activity in comparison with control plants indicating that oxidative stress took place. It is postulated that PQ may be associated with mechanisms maintaining a tightly controlled balance between the accumulation of ROS and antioxidant activity that determines the full expression of effective defence 139.
Somatic hybrids between the cultivated potato diploid hybrid clone, ZEL-1136, and hexaploid non-tuber-bearing wild species Solanum nigrum L. exhibiting resistance to Phytophthora infestans were regenerated after PEG-mediated fusion of mesophyll protoplasts. The most vigorous flowering somatic hybrids were selected for assessment of the late-blight resistance 140. Solanum nigrum L. (SNL) has been traditionally used as a herbal plant, whosefruit is believed to have anti-tumor properties, although the mechanism for the activity remains to be elucidated. The SNL extract was revealed to be a potential scavenger of hydroxyl radicals and DPPH radicals rather than superoxide anions. Collectively, our findings suggest that SNL fruit extract could be used as an anti-oxidant and cancer chemo-preventive material 141.
This study based on the plant Solanum nigrum and other plant species is involved in this process and all activities based on the plant and animal species. Alkaline phosphatase (ALP) and total bilirubin. The histopathological changes of liver sample in treated animals were compared with respect to control 142.
Molluscicidial activity of leaves of Acanthus mollis against Biomphalaria alexandrina was evaluated. Its petroleum ether extract (LC50 values = 6.92 mg/L) was more potent than Solanum nigrum and Iris pseudacorus extracts. A binary combination (1:1) of A. mollis and S. nigrum, as well as, a binary combination (1:1) of A. mollis and I. pseudacorus extracts showed additive effect on snails (24 hr LC50: 5.09 mg/l and 3.76 mg/l respecttively). A tertiary combination (1:1:1) of A. mollis, S. nigrum and I. pseudacorus extracts (24 hr LC50: 4.01 mg/l) showed good result. Also, petroleum ether extract of A. mollis leaves killed Schistosoma mansoni cercariae at concentrations of 20, 10 and 5 mg/l within 30, 45 min. and an hour respectively.
Mortality increased with increasing exposure time and concentration 143. Solanum nigrum L. (SNL) has been traditionally used as an herbal plant for a long time. In the present study, SNL glycoprotein showed a dose-dependent radical scavenging activity on radicals, including 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radicals, hydroxyl radical (OH), and superoxide anion (O (2) (-)). When the HT-29 cells were treated with 60μg/ml SNL glycoprotein, the cytotoxic effect was induced in a time-dependent manner. More specifically, according to the apoptosis assay, increased as a result of treatment with 40μg/ml SNL glycoprotein in a time-dependent manner, whereas they were weakly induced by GO in the cells. Consequently, the SNL glycoprotein may induce apoptosis through the inhibition of NF-κB activation, induced by oxidative stress in HT-29 cells 144.
Virus-induced gene silencing (VIGS) has been used routinely in Nicotiana benthamiana to assess functions of candidate genes and as a way to discover new genes required for diverse pathways, especially disease resistance signalling. VIGS has recently been shown to work in Arabidopsis thaliana and in tomato. Here, we report that VIGS using the tobacco rattle virus (TRV) viral vector can be used in several Solanum species,.
Silencing of R1, Rx and RB successfully attenuated R-gene-mediated disease resistance and resulted in susceptible phenotypes in detached leaf assays. Thus, the VIGS system is an effective method of rapidly assessing gene function in potato 145. This study was carried out to investigate the anticancer effects of a 150-kDa glycoprotein isolated from Solanum nigrum L. (SNL glycoprotein) on spontaneously and experimentally induced tumor promotion in HCT-116 cells. For spontaneously induced tumor promotion, dose-dependent cytotoxic and apoptosis-inducing effects at low concentrations. For experimentally induced tumor promotion collectively, these results suggest that SNL glycoprotein can induce apoptosis through the modulation of signal mediators. Therefore, we speculate that it could be used as a chemotherapy agent even at low concentrations in HCT-116 cells 146.
Screening was done of some plants of importance in the Ayurvedic system of traditional medicine used in India to treat enteric diseases. Fifty four plant extracts (methanol and aqueous) were assayed for their activity against multi-drug resistant Salmonella typhi. Strong antibacterial activity was shown by the methanol extracts of ten medicinal plants. Moderate antimicrobial activity was shown by Solanum nigrum and other herbs of interest 147.
This study was carried out to investigate the modulation of detoxicant enzyme activity and plasma lipidemic levels by 150 kDa glycoprotein isolated from Solanum nigrum Linn. (SNL), these results pointed out that SNL glycoprotein can enhance the activities of detoxicant enzymes and bring about the inhibition of HMG-CoA reductase activity in vivo. Therefore, we speculate that SNL glycoprotein can be used as a cholesterol-lowering agent even at low Concentration 148. Solanum nigrum L. (SNL) has been used in folk medicine for its anti-inflammatory activity. They isolated only the SNL glycoprotein from SNL and found that it was cytotoxic at low concentration.
The results in this study indicated that SNL glycoprotein induces apoptosis through modulation of PKCalpha and NF-kappaB activity in MCF-7 cells 149. A crude water extract of Solanum nigrum leaves was used as a chemical attenuate to Schistosoma mansoni cercariae prior to infection of Swiss female mice. Cercariae were exposed to 2.5, 5, 7.5 and 10 mg/l concentrations of the extract for 30 min. The effect on the ability of cercariae to penetrate mice skin, as well as, effect on schistosome worm burden after 8 weeks of infection were measured 150.
To study cytotoxicity and antineoplastic effect in vitro Solanum nigrum L extract on U266. U266 cells were cultured together with the extract of Solanum nigrum L. Cytotoxicity assay was tested by CCK-8. Cell cycle and apoptosis were determined using flow cytometry (FCM) analysis and cytotoxicity effect on U266 cells. The antineoplastic effect of the drug can partly be ascribed to its apoptotic inducing effect 151. Model systems have proven enormously useful in elucidating the biochemical function of plant genes. They analyzed transcriptional responses of two native Solanaceous species to the attack of an herbivore whose elicitors are known not to be influenced by diet.
Given that attack from the same herbivore elicits profoundly different responses in two Solanaceaous taxa, we conclude that blueprints for commonly regulated responses to plant-herbivore interactions appear unlikely 152. Nitric oxide (NO) is an antitumour molecule produced in activated macrophages and Solanum nigrum is a plant used in oriental medicine to treat tumours. In this study using mouse peritoneal macrophages, these findings suggest that Solanum nigrum increases the production of NO by rIFN-gamma-primed macrophages and NF-kappaB plays a critical role in mediating these effects 153.
This study was carried out to investigate apoptotic effects of the glycoprotein (SNL glycoprotein, 150 kDa) isolated from Solanum nigrum Linne, which has been used as an anti-pyretic and anti-cancer agent in folk medicine. We found that the SNL glycoprotein consists of carbohydrate (69.74%) and protein content (30.26%), which has >50% hydrophobic amino acids containing glycine and proline.
The results of this experiment suggest that the SNL glycoprotein activates caspase-3 in HT-29 cells, independent of ROS 154. Solanum nigrum L. (SNL) has been used in folk medicine for its anti-inflammatory activity. These results indicate that SNL glycoprotein causes HT-29 cell death through apoptosis by its ability to modulate anti-apoptotic signals. We suggest that SNL glycoprotein is a natural anti-cancer agent due to its potential to induce apoptosis in HT-29 cells 155.
This paper gave a brief introduction of the effect of Solanum nigrum on anti-cancer. The experimental results showed that the total alkaloid isolated from S. nigrum interfered structure and function of tumor cell membrane, disturbed the synthesis of DNA and RNA, while the glycoprotein (150 x 10(3)) isolated from S. nigrum might have shown anti-cancer abilities by blocking the anti-apoptotic pathway of NF-kappaB, activating caspase cascades reaction and increasing the production of nitric oxide 156.
The present study was carried out to evaluate the antinociceptive, anti-inflammatory and antipyretic effects of chloroform extract of Solanum nigrum leaves using various animal models. The present studies demonstrated that the lipid-soluble extract of S. nigrum leaves possessed antinociceptive, anti-inflammatory and anti-pyretic properties and confirmed the traditional claims 157. This study based on Solanum nigrum Linn. (SNL) has been traditionally used as herbal agent in folk medicine for various cancers in Korea. We found that the SNL glycoprotein consists of carbohydrate (69.74%) and protein content (30.26%), interestingly, and it may be a potential candidate in field of anticancer drug discovery 158.
Solanum nigrum L. (SN) has been used in traditional folk medicine to treat different cancers. These findings indicate that SNE induced cell death in hepatoma cells via two distinct antineoplastic activities of SNE, the ability to induce apoptosis and autophagocytosis, therefore suggesting that it may provide leverage to treat liver cancer 159. Solanum nigrum Linn. (SNL) has been used in traditional Chinese medicine for centuries because of its diuretic and antipyretic effects. The present study examined the effect of the crude polysaccharides isolated from Solanum nigrum Linne (SNL-P) on tumor growth. This resulted in a massive necrosis in tumor tissues and the up-regulation of Bax and down-regulation of Bcl-2 and mutant p53 gene expression, which triggered apoptosis in tumor cells. These findings demonstrated that the SNL-P is a potential antitumor agent 160.
Schistosomes have a complex lifecycle with freshwater intermediate host snails. The snail host represents the weakest point in the lifecycle of parasite.Biomphalaria arabica is intermediate host for Schistosoma mansoni in Saudi Arabia.The studied enzymes were altered in molluscicide-treated snails compared to control. AST and ALT were slightly affected but LDH was the most significantly altered enzyme.
The role of the biochemical manipulation in affecting host-parasite relationship was discussed 161.
To study the inhibitory effect of Solanum nigrum on angiogenesis. The surrounding CAM showed a few angiogenesis formation. However, in the control group, a number of angiogenesis were observed: S. nigrum could inhibit the angiogenesis on CAM 162. Solanum nigrum L. (SN) is an herbal plant that has been used as hepatoprotective and anti-inflammatory agent in Chinese medicine. The results of this study suggest that SNE could protect liver against the CCl4-induced oxidative damage in rats, and this hepatoprotective effect might be contributed to its modulation on detoxification enzymes and its antioxidant and free radical scavenger effects 163.
Bone undergoes continuous remodeling through bone formation and resorption, and maintaining the balance for skeletal rigidity. Bone resorption and loss are generally attributed to osteoclasts. Differentiation of osteoclasts is regulated by receptor activator of nuclear factor NF-kB ligand (RANKL), Among 222 methanol extracts, of medicinal plants, 10 samples exhibited ability to induce osteoclast differentiation 164. To explore the antitumor activity of aqueous extract of Solanum nigrum (SNL-AE) and its possible mechanism 165.
Antioxidant studies: Some useful study was based on the antioxidant identification in plant S.nigrum & other plant species. Ninety crude extracts, including dichloromethane, methanol and aqueous extracts from 30 medicinal plants used in the Yemeni ethnomedicine to treat common infections, and showed effective free radical scavenging activities in the DPPH assay 166. Some scincetist study based on antioxidant in three Solanum genotypes with various polygenic resistance levels to the oomycete pathogen Phytophthora infestans (Mont.) De Bary were studied for their antioxidant response to the pathogen culture filtrate 167.
Parasitic & Microbiological studies: Some scincetist study based on Biochemical analyses for pathogenicity achieved with attenuated cercariae showed that while serum aspartate and alanine aminotransferases (AST &ALT) were more or less similar, depleted glycogen and elevated lipid peroxides were normalized when compared to those infected with normal cercariae 168.Sciencetist studied on Molluscicidial activity of leaves of Acanthus mollis against Biomphalaria alexandrina were evaluated, S. nigrum and I. pseudacorus extracts (24 hr LC50: 4.01mg/l) showed good result. And an hour respectively. Mortality increased with increasing exposure time and concentration 169.
CONCLUSION: S. nigrum is wonderful plants having enormous range of medicinal activity in this article have assanbled almost all information related to different research activity of plant. Although it is mentioned as a component in several popular polyherbal formulations in the form of alcoholic or hydroalcoholic extracts, it is an attractive candidate plant for formulating targeted drugs. This review will help to researchers & scholars to go deep in this area as plant indicate vast range of phytochemical related to origin so it can be suggested the further work can be done on S. nigrum. This is collected from different season & agroclimatic zone.
Definitely, it is assumed that research will be able to find out more suitable & specific drug plant having particle activity in specific season. Same type of review paper has been published on Tribulus terrestris, a traditionally important wild medicinal herb of waste lands, which became a popular article for further investigations on particular medicinal herbs. All Some scientist these data and concepts are in need to re-research on the present scientific tools. It can really contribute to medical and pharmaceutical practices. There are still many more activities waiting for screening the drug 170.
- Trees GE, Evans MC: Text book of Pharmacognosy London 1983; 121: 343-383.
- 2. Mohy-ud-dint A, Khan Z, Ahmad M and Kashmiri M.A: Chemotaxonomic value of alkaloids in Solanum nigrum complex. Pakistan Journal of Botany 2010; 42(1): 653-660.
- Ahmed AH, Rifaat M: Effect of Solanum nigrum leaves water extract on the penetration and infectivity of schistiosoma cereariae. j Egypt soc parasitol 2008; 35:1:33-40.
- Venkateswarlu J, Krishna Rao M: Inheritance of fruit colour in the Solanum nigrum complex, Proceedings. Plant Sciences 2006; 74- 3:137-141.
- Turner N.J., Aderka P.von: The North American guide to common poisonous plants and mushrooms. Timber Press 2008; 181-182.
- Aslanov S.M: Glycoalkaloids of Solanum nigrum. Chemistry of Natural Compounds 1971; 658.
- Schep LJ, Slaughter RJ, and Temple WA: Notes on poisoning black nightshade". The New Zealand Medical Ca Journal 1970; 122 (1292):95–6.
- North P: Poisonous Plants and Fungi in Colour. Blandford Press 1977; 140-141.
- Sun RI, Zhou Q-x, and Wang X: Relationships between cadmium accumulation and organic acids in leaves of Solanum nigrum L. as a cadmium-hyperaccumulator .Huan jing ke xue 2006; 27(4): 765-769.
- Cooper MR, Johmson AW: Poisonous Plant in Britain and other effect on animals and Man. Ministry of agriculture Fisheries Food 1984; 161:219-220.
- Zhou X, He X, Wang G, Gao H, Zhou G,Ye W, Yao X: Steroidal saponins from Solanum nigrum. J Nat Prod 2006; 69(8):1158-1163.
- Jian L ,Qing-Wang L, Da-Wei G, Zeng-Sheng L: Antitumor and immunomodulating effect of polysaccharides isolated from Solanum nigrum Linn. Phytother Res 2009; 23(11):1524-1530.
- Muto M, Mulabagal V, Huang H-C, Takahashi H, Tsay H-S, Huang J-W: Toxicity of black nightshade (Solanum nigrum) extract on (Alternaria brassicicola, causal agent of black leaf spot of Chinese cabbage (Brassica pekinensis). J Phytopathol 2006; 154(1):45-50.
- Adebooye OC, Ram V, Vasudeva S: Peroxidase activity chlorophylls and antioxidant profile of two leaf vegetables (Solanum nigrum L.and Amaranthus cruentus L.) under six pretreatment methods before cooking. Int J Food Sci Technol 2008; 43(1):173-178.
- Raju K, Anbuganapathi G, Gokulakrishnan V, Rajkapoor B, Jayakar B, Maninan S: Effect of dried fruits of Solanum nigrum Linn against CCl4-induced hepatic damage in rats. Biol Pharm Bull 2003; 26 (11):1618-1619.
- Perez G, Perez L, Garcia D, Sossa M: Neuropharmacological activity of Solanum nigrum fruit. J.Ethnopharmacol 1998; 62(1):43-48.
- Gupta M, Mazumder UK, and Gomathi P, Thamilselvan V: Antiinflammatory evaluation of leaves of Plumeria acuminates BMC Complementary and alternative medicine. 2006; 6:36:1472-6882.
- Nohara T, Ikeda T, Fujiwara Y, Matsushita S, Noguchi E, Yoshimitsu H, Ono M: Physiological functions of solanaceous and tomato steroidal glycosides. Journal of Natural Medicines 2004; 1-13.
- Zemede Asfaw: "Conservation and use of traditional vegetables in Ethiopia".Proceedings of the IPGRI International Workshop on Genetic Resources of Traditional Vegetables in Africa 1995; 29–31.
- Jansen van Rensburg, WS et al.: “African leafy vegetables in South Africa”. Water SA 2007; 33(3):317–326.
- Schauenberg P, Paris F: Guide to Medicinal Plants. Keats Publishing Inc 1977; 53.
- Nohara T, Yahara S, Kinjo J: Bioactive Glycosides from Solanaceous and Leguminous Plants. Natural Product Sciences 1998; 4(4):203-214.
- Jai, SK: Medicinal Plants. Thomson Pess 1968; 133-134.
- Kaushik D, Jogpal1 V, Kaushik P, Lal S, Saneja A, Sharma C, Aneja K.R: Evaluation of activities of Solanum nigrum fruit extract. Archives of Applied Science Research 2009; 1 (1): 43- 47.
- Jian L, Qingwang L, Tao F, Kun L, Aqueous extract of Solanum nigrum inhibit growth of cervical carcinoma (U14) via modulating immune response of tumor bearing mice and inducing apoptosis of tumor cells. Fitoterapia 2008; 79(78):548-556.
- Jain R, Sharma A, Gupta S, Sarethy IP, Gabrani R"Solanum nigrum: current perspectives on therapeutic properties." Altern Med Rev 2011; 16(1):78-85.
- Goloubinoff P, Edelman M, and Hallick RB: Chloroplast-coded atrazine resistance in Solanum nigrum: psbA loci from susceptible and resistant biotypes are isogenic except for a single codon change. Nucleic Acids Res 1984; 12(24):9489-96.
- Zhu L, Hu N, Zhai W, Li X: [The nucleotide sequence of the chloroplast psbA gene from Solanum nigrum atrazine resistant biotype and its relevant analysis]. Yi Chuan Xue Bao 1989; 16(5):381-8.
- Havaux M: Stress Tolerance of Photosystem II in Vivo: Antagonistic Affects of Water, Heat and Photoinhibition Stresses. Plant Physiol 1992; 100(1):424-32.
- Narváez-Vásquez J, Orozco-Cárdenas ML, Ryan CA: Differential expression of a chimeric CaMV-tomato proteinase Inhibitor I gene in leaves of transformed nightshade, tobacco and alfalfa plants. Plant Mol Biol 1992; 20(6):1149-57.
- Johnson R, Lee JS, Ryan CA: Regulation of expression of a wound-inducible tomato inhibitor I gene in transgenic nightshade plants. Plant Mol Biol 1990; 14(3):349-56.
- Ye J, Sayre RT: Reduction of Chloroplast DNA Content in Solanum nigrum Suspension Cells by Treatment with Chloroplast DNA Synthesis Inhibitors. Plant Physiol 1990; 94:1477-83.
- Kavanagh TA, O'Driscoll KM, McCabe PF, and Dix PJ: Mutations conferring lincomycin, spectinomycin, and streptomycin resistance in Solanum nigrum are located in three different chloroplast genes. Mol Gen Genet 1994; 242(6):675-80.
- Kavanagh TA, Thanh ND, Lao NT, McGrath N, Peter SO, Horváth EM, and Dix PJ, Medgyesy P: Homeologous plastid DNA transformation in tobacco is mediated by multiple recombination events. Genetics 1999; 152(3):1111-22.
- Ehlers K, Kollmann R: Synchronization of mitotic activity in protoplast-derived Solanum nigrum L.microcalluses is correlated with lasmodesmal connectivity. Planta 2000; 210:269-78.
- Polkowska-Kowalczyk L, Maciejewska U: The oxidative processes induced in cell suspensions of Solanum species by culture filtrate of Phytophthora infestans. Z Naturforsch C 2001; 56(3-4):235-44.
- Mansouri H, Petit A, Oger P, Dessaux Y: Engineered rhizosphere: the trophic bias generated by opine-producing plants is independent of the opine type, the soil origin, and the plant species. Appl Environ Microbiol 2002; 68(5):2562-6.
- Scott IM, Jensen H, Scott JG, Isman MB, Arnason JT, Philogène BJ: Botanical insecticides for controlling agricultural pests: piperamides and the Colorado Potato, Beetle Leptinotarsa decemlineata say(Coleoptera Chrysomelidae). Arch Insect Biochem Physiol 2003; 54(4):212-25.
- Zimnoch-Guzowska E, Lebecka R, Kryszczuk A, Maciejewska U, Szczerbakowa A,Wielgat B: Resistance to Phytophthora infestans in somatic hybrids of Solanum nigrum L. and diploid potato. Theor Appl Genet 2003; 107(1):43-8.
- Polkowska-Kowalczyk L, Wielgat B, Maciejewska U: The elicitor-induced oxidative processes in leaves of Solanum species with differential polygenic resistance to Phytophthora infestans. J Plant Physiol 2004; 161(8):913-20.
- Nugent GD, Ten Have M, van der Gulik A, Dix PJ, Uijtewaal BA, Mordhorst AP: Plastid transformants of tomato selected using mutations affecting ribosome structure. Plant Cell Rep 2005; 24(6):341-9.
- Guo SL, Fang F, Ni L, and Chen W, Shi L: [Photosynthetic characteristics and coenological survey of Lactuca serriola in its invaded area]. Ying Yong Sheng Tai Xue Bao 2006; 17(12):2316-20.
- Schmidt S, Baldwin IT: Systemin in Solanum nigrum. The tomato-homologous polypeptide does not mediate direct defense responses. Plant Physiol 2006; 142(4):1751-8.
- Riethmuller-Haage I, Bastiaans L, Harbinson J, Kempenaar C, and Kropff MJ: Influence of the acetolactate synthase inhibitor metsulfuron-methyl on the operation, regulation and organisation of photosynthesis in Solanum nigrum. Photosynth Res 2006; 88(3):331-41.
- Katsan VA, Potopal's'kyĭ AI: [Changed ratios of some pigments of photosynthesis in Nicotiana tabacum L.induced by exogenic DNA]. Ukr Biokhim Zh 2006; 78(5):70-80.
- Jami SK, Swathi Anuradha T, Guruprasad L, Kirti PB: Molecular, biochemical and structural characterization of osmotin-like protein from black nightshade (Solanum nigrum). J Plant Physiol 2007; 164(3):238-52.
- Polkowska-Kowalczyk L, Montillet JL, Agnel JP, Triantaphylidès C, Wielgat B, Maciejewska U: Changes in the initial phase of lipid eroxidation induced by elicitor from Phytophthora infestans in Solanum species. J Plant Physiol 2008; 165(18):1929-39.
- Campos Mde A, Silva MS, Magalhães CP, Ribeiro SG, Sarto RP, Vieira EA, Grossi de Sá MF: Expression in Escherichia coli, purification, refolding and antifungal activityof an osmotin from Solanum nigrum. Microb Cell Fact 2008; 11:7:7.
- Rezek J, Macek T, Mackova M, Triska J: Plant mEtabolites of polychlorinated biphenyls in hairy root culture of black nightshade Solanum nigrum SNC-9O. Chemosphere 2007; 69(8):1221-7.
- Dijkstra C, Adams E, Bhattacharya A, Page AF, Anthony P, Kourmpetli S, Power JB, Lowe KC, Thomas SG, Hedden P, Phillips AL, Davey MR: Over-expression of a gibberellin 2-oxidase gene from Phaseolus coccineus L.enhances gibberellin inactivation and induces dwarfism in Solanum species. Plant Cell Rep 2008; 27(3):463-70
- Bhat MA, Ahmad S, Aslam J, Mujib A, Mahmooduzzfar: Salinity stress enhances production of solasodine in Solanum nigrum L. Chem Pharm Bull (Tokyo) 2008; 56(1):17-21.
- Wu XF, Shi HP, Tsang P, Keung E: [Induction and in vitro culture of hairy roots of Solanum nigrum L.var. pauciflorum Liou and its solasodine production]. Fen Zi Xi Bao Sheng Wu Xue Bao 2008; 41(3):183-91.
- Hartl M, Giri AP, Kaur H, and Baldwin IT: Serine protease inhibitors specifically defend Solanum nigrum against generalist herbivores but do not influence plant growth and development. Plant Cell 2010; 22(12):4158-75.
- De Cauwer B, Van den Berge K, Cougnon M, Bulcke R, Reheul D: Weed seed bank response to 12 years of different fertilization systems. Commun Agric Appl Biol Sci 2010; 75(2):61-72.
- Bajkán S, Váradi G, Balogh M, Domonkos A, Kiss GB, Kovács L, Lehoczki E: Conserved structure of the chloroplast-DNA encoded D1 protein is essential for effective photoprotection via non-photochemical thermal dissipation in higher plants.Mol Genet Genomics 2010; 284(1):55-63.
- Gotoh T, Sugimoto N, Pallini A, Knapp M, Hernandez-Suarez E, Ferragut F, Ho CC, Migeon A, Navajas M, Nachman G: Reproductive performance of seven strains of the tomato red spider mite Tetranychus evansi (Acari: Tetranychidae) at five temperatures. Exp Appl Acarol 2010; 52(3):239-59.
- Gao Y, Miao C, Mao L, Zhou P, Jin Z, Shi W: Improvement of phytoextraction and antioxidative defense in Solanum nigrum L.under cadmium stress by application of cadmium-resistant strain and citric acid. J Hazard Mater 2010; 181(1-3):771-7.
- Poczai P, Cernák I, Gorji AM, Nagy S, Taller J, Polgár Z: Development of intron targeting (IT) markers for potato and cross-species amplification in Solanum nigrum (Solanaceae). Am J Bot 2010; 97(12):142-5.
- Marques AP, Oliveira RS, Samardjieva KA, Pissarra J, Rangel AO, Castro PM: Solanum nigrum grown in contaminated soil: effect of arbuscular mycorrhizal fungi on zinc accumulation and histolocalisation.Environ Pollut 2007; 145(3):691-9.
- Kadioğlu I, Karamanli N, Yanar Y: Determination of fungal pathogens of common weed species in the vicinity of Tokat, Turkey. Commun Agric Appl Biol Sci 2010; 75(2):97-105.
- VanDoorn A, Bonaventure G, Schmidt DD, Baldwin IT: Regulation of jasmonate metabolism and activation of systemic signaling in Solanum nigrum. New Phytol 2011; 190(3):640-52.
- Poczai P, Hyvönen J: Ye W44On the origin of Solanum nigrum can network help. Mol Biol Rep 2011; 38(2):1171-85.
- Veiga RS, Jansa J, and Frossard E, van der Heijden MG: Can arbuscular mycorrhizal fungi reduce the growth of agricultural weeds? PLoS One 2011; 6(12): 278-25.
- Bhattacharya A, Ward DA, Hedden P, Phillips AL, Power JB, and Davey MR: Engineering gibberellin metabolism in Solanum nigrum L. by ectopic expression of gibberellin oxidase genes. Plant Cell Rep 2012.
- Kloos H, Thiongo FW, Ouma JH, Butterworth AE: Preliminary evaluation of some wild and cultivated plants for snail control in Machakos District, Kenya. J Trop Med Hyg 1987; 90(4):197-204.
- 66. Schmidt DD, Kessler A, Kessler D, Schmidt S, Lim M, Gase K, Baldwin IT: Solanum nigrum: a model ecological expression system and its tools. Mol Ecol 2004; 13(5):81-95.
- Kazinczi G, Takács AP, Horváth J, and Gáborjányi R, Béres I: Susceptibility of some weeds species to Pepino mosaic virus (PepMV). Commun Agric Appl Biol Sci 2005; 70(3):489-91.
- Kadioglu I, Yanar Y, Asav U: Allelopathic effects of weeds extracts against seed germination of some plants. J Environ Biol 2005; 26. (2):169-73.
- Wei SH, Zhou QX, Wang X: [Cadmium-hyperaccumulator Solanum nigrum L. and its accumulating characteristics]. Huan Jing Ke Xue 2005; 26(3): 167-71.
- Sun RL, Zhou QX, and Wang X: [Relationships between cadmium accumulation and organic acids in leaves of Solanum nigrum L. as a cadmium-hyperaccumulator] Huan Jing Ke Xue 2006; 27(4):765-9.
- Marques AP, Oliveira RS, Rangel AO, Castro PM: Zinc accumulation in Solanum nigrum is enhanced by different arbuscular mycorrhizal fungi. Chemosphere 2006; 65(7):1256-63.
- Verma M, Rao PB: Allelopathic effect of four weed species extracts on germination, growth and protein in different varieties of Glycine max (L.) Merrill. J Environ Biol 2006; 27(3):571-7.
- Peng K, Li X, Luo C, Shen Z: Vegetation composition and heavy metal uptake by wild plants at three contaminated sites in Xiangxi area, China. J Environ Sci Health A Tox Hazard Subst Environ Eng 2006; 41(1):65-76.
- Calvelo Pereira R, Camps-Arbestain M, and Rodríguez Garrido B, Macías F: Monterroso Behaviour of alpha-, beta-, gamma-, and delta-hexachlorocyclohexane in the soil-plant system of a contaminated site. ENviron Pollut 2006; 144(1):210-7.
- Zhou X, He X, Wang G, Gao H, Zhou G, Ye W, Yao X: Steroidal saponins from Solanum nigrum. J Nat Prod 2006; 69(8):1158-63.
- Abbaspoor M, Streibig JC: Monitoring the efficacy and metabolism of phenylcarbamates in sugar beet and black nightshade by chlorophyll fluorescence parameters. Pest Manag Sci 2007; 63(6):576-85.
- Grossmann K, Ehrhardt T: On the mechanism of action and selectivity of the corn herbicide topramezone: a new inhibitor of 4-Hydroxyphenylpyruvate dioxygenase. Pest Manag Sci 2007; 63(5):429-39.
- Wang L, Zhou Q, Ding L, Sun Y: Effect of cadmium toxicity on nitrogen metabolism in leaves of Solanum nigrum L. as a newly found cadmium hyperaccumulator. J Hazard Mater 2008; 15:154(1-3):818-25.
- Marques AP, Oliveira RS, Samardjieva KA, Pissarra J, Rangel AO, Castro PM: EDDS and EDTA-enhanced zinc accumulation by Solanum nigrum inoculated with arbuscular mycorrhizal fungi grown in contaminated soil. Chemosphere 2008; 70(6):1002-14.
- Cayuela ML, Millner PD, Meyer SL, Roig A: Potential of olive mill waste and compost as biobased pesticides against weeds, fungi, and nematodes. Sci Total Environ 2008; 399(1-3):11-8.
- Marques AP, Oliveira RS, Rangel AO, Castro PM: Application of manure and compost to contaminated soils and its effect on zinc accumulation by Solanum nigrum inoculated with arbuscular mycorrhizal fungi. Environ Pollut 2008; 151(3):608-20.
- Schmidt S, Baldwin IT: Down-regulation of systemin after herbivory is associated with increased root allocation and competitive ability in Solanum nigrum. Oecologia 2009; 159(3):473-82.
- Sun Y, Zhou Q, Wang L, Liu W: The influence of different growth stages and dosage of EDTA on Cd uptake and accumulation in Cd-hyperaccumulator (Solanum nigrum L.). Bull Environ Contam Toxicol 2009; 82(3):348-53.
- Wei S, Hu Y, Srivastava M, Zhou Q, Niu R, Li Y, Wu Z, Sun T: Seed germination of a newly discovered hyperaccumulator Solanum nigrum L.affected by illumination and seed-soaking reagent. J Hazard Mater 2009; 170(2-3):1256-9.
- Luo P, Niu H, Zheng G, Bai X, Zhang M, Wang W: From salmon pink to blue natural sensitizers for solar cells: Canna indica L.Salvia plendens, cowberry and Solanum nigrum L.Spectrochim Acta A Mol Biomol Spectrosc 2009; 74(4):936-42.
- Bao T, Sun L, Sun T, Zhang P, and Niu Z: Iron-deficiency induces cadmium uptake and accumulation in Solanum nigrum L. Bull Environ Contam Toxicol 2009; 82(3):338-42.
- Peng KJ, Luo CL, Chen YH, Wang GP, Li XD, Shen ZG: Cadmium and other metal uptake by Lobelia chinensis and Solanum nigrum from contaminated soils. Bull Environ Contam Toxicol 2009; 83(2):260-4.
- Vrkoslavová J, Demnerová K, Macková M, Zemanová T, Macek T, Hajslová J, Pulkrabová J, and Hrádková P, Stiborová H: Absorption and translocation of polybrominated diphenyl ethers (PBDEs) by plants from contaminated sewage sludge. Chemosphere 2010; 81(3):381-6.
- Xu J, Yin H, Liu X, and Li X: Salt affects plant Cd-stress responses by modulating growth and Cd accumulation. Planta 2010; 231(2):449-59.
- Xiao X, Luo S, Zeng G, Wei W, Wan Y, Chen L, Guo H, Cao Z, Yang L, Chen J, Xi Q: Biosorption of cadmium by endophytic fungus (EF) Microsphaeropsis sp. LSE10 isolated from cadmium hyperaccumulator Solanum nigrum L. Bioresour Technol 2010; 101(6):1668-74.
- Shi H, Tsang EP, and Wang Y, Chan AL: [Affect of cadmium, alone or in combination with CaCl2, on the growth, antioxidative enzyme activity and cadmium absorption of Solanum nigrum L. Var pauciflorum hairy roots]. Sheng Wu Gong Cheng Xue Bao 2010; 26(2):147-5852.
- Deng X, Xia Y, Hu W, Zhang H, Shen Z: Cadmium-induced oxidative damage and protective effects of N-acetyl-L-cysteine against cadmium toxicity in Solanum nigrum L. J Hazard Mater 2010; 180(1-3):722-51.
- Wei S, Li Y, Zhou Q, Srivastava M, Chiu S, Zhan J, Wu Z, Sun T: Effect of fertilizer amendments on phytoremediation of Cd-contaminated soil by a newly discovered hyperaccumulator Solanum nigrum L. J Hazard Mater 2010; 176(1-3):269-73.
- Kumar N, Chaturvedi SS, Jha SK: Assessment of doses and risk due to natural radonuclides in edible biota of domiasiat Meghalaya. Radiat Prot Dosimetry 2011.
- Luo S, Wan Y, Xiao X, Guo H, Chen L, Xi Q, Zeng G, Liu C, Chen J: Isolation and characterization of endophytic bacterium LRE07 from cadmium hyperaccumulator Solanum nigrum L. and its potential for remediation. Appl Microbiol Biotechnol 2011; 89(5):1637-4431.
- Yang C, Zhou Q, Wei S, Hu Y, Bao Y: Chemical-assisted phytoremediation of CD-PAHs contaminated soils using Solanum nigrum L. Int J Phytoremediation 2011; 13(8):818-33.
- Ji P, Song Y, Sun T, Liu Y, Cao X, Xu D, Yang X, McRae T: In-situ cadmium phytoremediation using Solanum nigrum L.the bio-accumulation characteristics trail.Int J Phytoremediation 2011; 13(10):1014-23.
- Luo SL, Chen L, Chen JL, Xiao X, Xu TY, Wan Y, Rao C, Liu CB, Liu YT, Lai C, and Zeng GM: Analysis and haracterization of cultivable heavy metal-resistant bacterial endophytes isolated from Cd-hyperaccumulator Solanum nigrum L. and their potential use for phytoremediation. Chemosphere 2011; 85(7):1130-8.
- Vandoorn A, Bonaventure G, Rogachev I, Aharoni A, Baldwin IT JA-Ile: Signalling in Solanum nigrum is not required for defence responses in nature.Plant Cell Environ 2011; 34(12):2159-71.
- Luo S, Xu T, Chen L, Chen J, Rao C, Xiao X, Wan Y, Zeng G, Long F, Liu C, Liu Y: Endophyte-assisted promotion of biomass production and metal-uptake of energy crop sweet sorghum by plant-growth-promoting endophyte Bacillus sp. SLS18. Appl Microbiol Biotechnol 2012; 93(4):1745-5318.
- Dafni A, Yaniv Z: Solanaceae as medicinal plants in Israel. J Ethnopharmacol 1994; 44(1):11-8.
- Perez RM, Perez JA, Garcia LM, Sossa H: Neuropharmacological activity of Solanum nigrum fruit. J Ethnopharmacol 1998; 62(1):43-8.
- Ahmed AH, Kamal IH, Ramzy RM: Studies on the molluscicidal and larvicidal properties of Solanum nigrum L. leaves ethanol extract. J Egypt Soc Parasitol 2001; 31(3):843-52.
- Agarwal AR, Gahlot A, Verma R, and Rao PB: Effect of weed extracts on seedling growth of some varieties of wheat. J Environ Biol 2002; 23(1):19-23.
- Cavlovic P, Mankotia M, and Pantazopoulos P, Scott PM: Liquid chromatographic etermination of alpha-solasonine in frozen green peas as an indicator of the presence of nightshade berriesJ AOAC Int 2003 86(4):759-63.
- Hebbar SS, Harsha VH, Shripathi V, Hegde GR: Ethnomedicine of Dharwad district in Karnataka, India--plants used in oral health care. J Ethnopharmacol 2004; 94(2-3): 261-6.
- Al-Qura'n S: Ethnopharmacological survey of wild medicinal plants in Showbak. J Ethnopharmacol 2009; 123(1):45-50.
- Mutheeswaran S, Pandikumar P, Chellappandian M, Ignacimuthu S: Documentation and quantitative analysis of the local knowledge on medicinal plants among traditional Siddha healers in Virudhunagar district of Tamil Nadu, India. J Ethnopharmacol 2011; 137(1):523-33.
- Hu K, Kobayashi H, and Dong A, Jing Y, Iwasaki S, and Yao X: Antineoplastic agents. III: Steroidal glycosides from Solanum nigrum. Planta Med 1999; 65(1):35-8.
- Ikeda T, Tsumagari H, Nohara T: Steroidal oligoglycosides from Solanum nigrum. Chem Pharm Bull (Tokyo) 2000; 48(7):1062-4.
- el-Ansary A, Elham, Sammour M, Mohamed AM: Susceptibility of Biomphalaria alexandrina to infection with Schistosoma mansoni: correlation with the activity of certain glycolytic enzymes. J Egypt Soc Parasitol 2000; 30(2):547-60.
- Sreevidya N, Mehrotra S: Spectrophotometric method for estimation of alkaloids precipitable with Dragendorff's reagent in plant materials. J AOAC Int 2003; 86(6):1124-7.
- Montillet JL, Cacas JL, Garnier L, Montané MH, Douki T, Bessoule JJ, Polkowska-Kowalczyk L, Maciejewska U, Agnel JP, Vial A, Triantaphylidès C: The upstream oxylipin profile of Arabidopsis thaliana: a tool to scan for oxidative stresses . Plant J 2004; 40(3):439-51.
- Cui S, Zhou Q, Chao L: [Absorption and accumulation of heavy metals by plants around a smelter]. Ying Yong Sheng Tai Xue Bao 2006; 17(3):512-5.
- Marques AP: Pregnane glycosides from Solanum nigrum. J Asian Nat Prod Res 2007; 9(6-8):517-23.
- Wang LY, Wang NL, Yao XS: [Non-saponins from Solanum nigrum. Zhong Yao CAI 2007; 30(7):792-4.
- Afolayan AJ, Jimoh FO: Nutritional quality of some wild leafy vegetables in South Africa. Int J Food Sci Nutr 2008; 1-8.
- Halim VA, Muck A, Hartl M, Ibáñez AJ, Giri A, Erfurth F, Baldwin IT, Svatos A: A dual fluorescent/MALDI chip platform for analyzing enzymatic activity and for protein profiling. Proteomics 2009; 9(1):171-81.
- Pearce G, Bhattacharya R, Chen YC, Barona G, Yamaguchi Y, and Ryan CA: Isolation and characterization of hydroxyproline-rich glycopeptide signals in black nightshade leaves. Plant Physiol 2009; 150(3):1422-33.
- Chen R, Feng L, Li HD, Zhang H, Yang F: Two novel oligosaccharides from Solanum nigrum. Carbohydr Res 2009; 344(13):1775-7.
- Yang MY, Hsu LS, Peng CH, Shi YS, Wu CH, and Wang CJ: Polyphenol-rich extracts from Solanum nigrum attenuated PKC alpha-mediated migration and invasion of hepatocellular carcinoma cells. J Agric Food Chem 2010; 58(9):5806-14.
- Zhao Y, Liu F, Lou HX: [Studies on the chemical constituents of Solanum nigrum]. Zhong Yao CAI 2010; 33(4):555-6.
- Wang L, Gao G, Bai Y, Luo W, and Lin C, Jia Q: Fingerprint quality detection of Solanum nigrum using high-performance liquid chromatography-evaporative light scattering detection on of the gastric antiulcerogenic effects of Solanum nigrum, Brassica oleracea and Ocimum basilicum in rats. J Ethnopharmacol 1989; 27(1-2):163-76.
- Yuan H, Chen Y, Cai B, Jia X, Chen Y: Taizhou [Simultaneous determination of three steroidal alkaloids from Solanum nigrum by RP-HPLC] . Zhongguo Zhong Yao Za Zhi 2011; 36(12):1630-2.
- Ohno M, Murakami K, El-Aasr M, Zhou JR, Yokomizo K, Ono M, Nohara T: New spirostanol glycosides from Solanum nigrum and S. jasminoides. J Nat Med 2012; 860-0082.
- Qureshi S, Rai MK, Agrawal SC: In vitro evaluation of inhibitory nature of extracts of 18-plant species of Chhindwara against 3-keratinophilic fungi. Hindustan Antibiot Bull 1997; 39(1-4):56-60.
- Constabel CP, Yip L, Ryan CA; Prosystemin from potato, black nightshade, and bell pepper primary structure and biological activity of Predicted systemin polypeptides. Plant Mol Biol 1998; 36(1):55-62.
- Abbas HK, Paul RN, Riley RT, Tanaka T, Shier WT: Ultrastructural effects of AAL-toxin TA from the fungus Alternaria alternata on black Nightshade (Solanum nigrum L.) leaf discs and correlation with biochemical measures of toxicity.Toxicon 1998; 36(12):1821-32.
- Ahmed AH, Ramzy RM: Seasonal variation in molluscicidal activity of Solanum nigrum L. J Egypt Soc Parasitol 1998; 28(3):621-9.
- Sammon AM: Protease inhibitors and carcinoma of the esophagus. Cancer 1998; 83(3):405-8.
- Ahmed AH, Ramzy RM: Laboratory assessment of the molluscicidal and cercaricidal activities of the Egyptian weed, Solanum nigrum L. Ann Trop Med Parasitol 1997; 91(8):931-7.
- Sultana S, Perwaiz S, and Iqbal M, Athar M: Crude extracts of hepatoprotective plants, Solanum nigrum and Cichorium intybus inhibit free radical-mediated DNA damage. J Ethnopharmacol 1995; 45(3):189-92.
- Sammon AM: A case-control study of diet and social factors in cancer of the esophagus in Transkei. Cancer 1992; 69(4):860-5.
- Moundipa PF, Domngang FM: Effect of the leafy vegetable Solanum nigrum on the activities of some liver drug-metabolizing enzymes after aflatoxin B1 treatment in female rats. Br J Nutr 1991; 65(1):81-91.
- Akhtar MS, Munir M: Evaluati. Pharm Biol 2011; 49(6):595-601.
- Savchenko T, Whiting P, Germade A, Dinan L: Ecdysteroid agonist and antagonist activities in species of the Solanaceae. Biochem Syst Ecol 2000; 28(5):403-419.
- Prashanth Kumar V, Shashidhara S, Kumar MM, Sridhara BY: Cytoprotective role of Solanum nigrum against gentamicin-induced kidney cell (Vero cells) damage in vitro. Fitoterapia 2001; 72(5):481-6.
- Yen GC, Chen HY, Peng HH: Evaluation of the cytotoxicity, mutagenicity and antimutagenicity of emerging edible plants. Food Chem Toxicol 2001; 39(11):1045-53.
- Maciejewska U, Polkowska-Kowalczyk L, and Swiezewska E, Szkopinska A: Plastoquinone: possible involvement in plant disease resistance. Acta Biochim Pol 2002; 49(3):775-80.
- Szczerbakowa A, Maciejewska U, and Zimnoch-Guzowska E, Wielgat B: Somatic hybrids Solanum nigrum (+) S. tuberosum: morphological Assessment and verification of hybridity. Plant Cell Rep 2003; 21(6):577-84.
- Son YO, Kim J, Lim JC, Chung Y, Chung GH, and Lee JC: Ripe fruit of Solanum nigrum L. inhibits cell growth and induces apoptosis in MCF-7 cells. Food Chem Toxicol 2003; 41(10):1421-8.
- Raju K, Anbuganapathi G, Gokulakrishnan V, Rajkapoor B, Jayakar B, Manian S: Effect of dried fruits of Solanum nigrum Linn.against CCl4-induced hepaticdamage in rats. Biol Pharm Bull 2003; 26(11):1618-9.
- Ahmed AH, Rifaat MM: Molluscicidal and cercaricidal efficacy of Acanthus mollis and its binary and tertiary combinations with Solanum nigrum and Iris pseudacorus against iomphalaria alexandrina. J Egypt Soc Parasitol 2004; 34(3):1041-50.
- Heo KS, Lee SJ, and Lim KT: Cytotoxic effect of glycoprotein isolated from Solanum nigrum L. through the inhibition of hydroxyl radical-induced DNA-binding activities of NF-kappa B in HT-29 cells. Environ Toxicol Pharmacol 2004; 17(1)45-54.
- Brigneti G, Martín-Hernández AM, Jin H, Chen J, Baulcombe DC, Baker B, Jones JD: Virus-induced gene silencing in Solanum species. Plant J 2004; 39(2)264-72.
- Lee SJ, Oh PS, Ko JH, Lim K, and Lim KT: A 150-kDa glycoprotein isolated from Solanum nigrum L. has cytotoxic and apoptotic effects by inhibiting the effects of protein kinase C alpha, nuclear factor-kappa B and inducible nitric oxide in HCT-116 cells. Cancer Chemother Pharmacol 2004; 54(6): 562-72.
- Rani P, Khullar N: Antimicrobial evaluation of some medicinal plants for their anti-enteric potential against multi-drug resistant Salmonella typhi. Phytother Res 2004; 18(8):670-3.
- Lee SJ, Ko JH, Lim K, Lim KT: 150 kDa glycoprotein isolated from Solanum nigrum Linne enhances activities of detoxicant enzymes and lowers plasmic cholesterol in mouse. Pharmacol Res 2005; 51(5):399-408.
- Heo KS, Lim KT: Glycoprotein isolated from Solanum nigrum L. modulates the apoptotic-related signals in 12-O-tetradecanoylphorbol 13-acetate-stimulated MCF-7 cells. J Med Food 2005; 8(1):69-77.
- Ahmed AH, Rifaat MM: Effects of Solanum nigrum leaves water extract on the penetration and infectivity of Schistosoma mansoni cercariae. J Egypt Soc Parasitol 2005; 35(1)33-40.
- Wang W, Lu DP: [An in vitro study of cytotoxic and antineoplastic effect of Solanum nigrum L extract on U266]. Beijing Da Xue Xue Bao 2005; 37(3):240-4.
- Schmidt DD, Voelckel C, Hartl M, Schmidt S, Baldwin IT: Specificity in ecological interactions: attack from the same lepidopteran herbivore results in species-specific transcriptional responses in two solanaceous host plants. Plant Physiol 2005; 138(3)1763-73.
- An HJ, Kwon KB, Cho HI, Seo EA, Ryu DG, Hwang WJ, Yoo SJ, Kim YK, Hong SH, Kim HM: Solanum nigrum produces nitric oxide via nuclear factor-kappaB activation in mouse peritoneal macrophages. Eur J Cancer Prev 2005; 14(4):345-50.
- Lee SJ, Lim KT: Apoptosis induced by glycoprotein (150-kDa) isolated from Solanum nigrum L. It is not related to intracellular reactive oxygen species (ROS) in HCT-116 cells. Cancer Chemother Pharmacol 2006; 57(4):507-16.
- Lim KT: Glycoprotein isolated from Solanum nigrum L. kills HT-29 cells through apoptosis. J Med Food 2005; 8(2):215-26.
- An L, Tang JT, Liu XM, and GAO NN: [Review about mechanisms of anti-cancer of Solanum nigrum]. Zhongguo Zhong Yao Za Zhi 2006; 31(15):1225-6, 1260.
- Zakaria ZA, Gopalan HK, Zainal H, and Mohd Pojan NH, Morsid NA, Aris A, Sulaiman MR: Antinociceptive, anti-inflammatory and antipyretic effects of Solanum nigrum chloroform extract in animal models. Yakugaku Zasshi 2006; 126(11):1171-8.
- Oh PS, Lim KT: HeLa cells treated with phytoglycoprotein (150 kDa) were killed by activation of caspase 3 via inhibitory activities of NF-kappaB and AP-1. J Biomed Sci 2007; 4(2):223-32.
- Lin HM, Tseng HC, Wang CJ, Chyau CC, Liao KK, Peng PL, Chou FP: Induction of autophagy and apoptosis by the extract of Solanum nigrum Linn in HepG2 cells. J Agric Food Chem 2007; 55(9):3620-8.
- Li J, Li Q, Feng T, Zhang T, Li K, Zhao R, Han Z, and Gao D: Antitumor activity of crude polysaccharides isolated from Solanum nigrum Linne on U14 cervical carcinoma bearing mice. Phytother Res 2007; 21(9):832-40.
- El-Ansary AK, Al Daihan SK: Effect of sublethal concentration of Solanum nigrum on transaminases and lactate dehydrogenase of Biomphalria arabica, in Saudi Arabia. J Egypt Soc Parasitol 2007; 37(1):39-50.
- Xu Y, Pan RL, Chang Q, Qin M, Liu Y, Tang JT: [Experimental study of Solanum nigrum on inhibiting angiogenesis in chick chorioallantoic membrane] Zhongguo Zhong Yao Za Zhi 2008; 33(5):549-52.
- Lin HM, Tseng HC, Wang CJ, Lin JJ, Lo CW, Chou FP: Hepatoprotective effects of Solanum nigrum Linn extract against CCl(4)-induced oxidative damage in rats . Chem Biol Interact 2008; 171(3):283-9.
- Youn YN, Lim E, Lee N, Kim YS, Koo MS, Choi SY: Screening of Korean medicinal plants for possible osteoclastogenesis effects in vitro. Genes Nutr 2008; 2(4):375-80.
- Li J, Li Q, Feng T, and Li K: Aqueous extract of Solanum nigrum inhibit growth of cervical carcinoma (U14) via modulating immune response of tumor bearing mice and inducing apoptosis of tumor cells. Fitoterapia 2008; 79(7-8):548-56.
- Al-Fatimi M, Wurster M, and Schröder G, Lindequist U: Antioxidant, antimicrobial and cytotoxic activities of selected medicinal plants from Yemen.J Ethnopharmacol 2007; 111(3):657-66.
- Polkowska-Kowalczyk L, Wielgat B, Maciejewska U: Changes in the antioxidant status in leaves of Solanum species in response to elicitor from Phytophthora infestans. J Plant Physiol 2007; 164(10):1268-77.
- El-Ansary A, Mohamed AM, and Mahmoud SS, El-Bardicy S: On the pathogenicity of attenuated Schistosoma mansoni cercariae released from metabolically disturbed Biomphalaria alexandrina. J Egypt Soc Parasitol 2003; 33(3):777-94.
- Ahmed AH, Rifaat MM: Molluscicidal and cercaricidal efficacy of Acanthus mollis and its binary and tertiary combinations with Solanum nigrum and Iris pseudacorus against Biomphalaria alexandrina. J Egypt Soc Parasitol 2004; 34(3)1041-50.
- Verma N, Kumar A, Saggoo MIS, Kumar V Gokharu (Tribulus terrestris L); a traditionally important wild medicinal herb of waste lands. Journal of plant development science 2009 vol: 1 (3 & 4) 179-187.
How to cite this article:
Kumar A, Sagwal S, Niketa and Rani S: An Updated Review on Molecular Genetics, Phytochemistry, Pharmacology and Physiology of Black Nightshade (Solanum nigrum). Int J Pharm Res Sci. 3(9); 2956-2977.
Ashwani Kumar*, S. Sagwal, Niketa and S. Rani
Department of Bioscience, Shri Ram College Muzaffarnagar, Uttar Pradesh, India
14 May, 2012
11 June, 2012
17 August, 2012
01 September, 2012