ANALGESIC ACTIVITY AND PHYTOCHEMICAL PROFILE OF AQUEOUS, ETHANOL AND DICHLOROMETHANE EXTRACTS OF PERSEA SCHIEDEANA LEAVESAbstract
Traditionally Persea schiedeana is used for headache relief, as anti-hypertensive, nervous relaxant, dermal diseases, among others. In this study, this plant from Salvadorian flora acute toxicity was evaluated in-vivo at 500 mg/kg, and the analgesic activity was tested by acetic acid, formalin, and tail flick tests in NIH Swiss mice at doses of 100, 250, and 500 mg/kg body weight, using as a positive control Indomethacin 10 mg/kg and distilled water as a control. Secondary metabolite profile was performed by Thin Layer Chromato-graphy (TLC) and ultra-efficient liquid chromatography-mass spectrometry (UPLC-MS). It was determined by TLC, the presence of terpenoids, flavonoids, tannins, lactonic groups, and coumarins, while UPLC-MS confirmed the presence of scopoletin. The biological assays demonstrated that P. schiedeana was deprived from toxic effect at the doses tested and the analgesic activity was confirmed in aqueous, ethanol, and dichloromethane extracts of Persea schiedeana leaves. Additionally, it was detected the presence of terpenoids, flavonoids, condensed tannins, lactones, and coumarins, and by UPLC-MS, the identity of coumarin as scopoletin was confirmed. These results are in accordance with the traditional use for pain relief.
J. G. Mejía, S. Vásquez, R. Salazar, L. Muñoz, U. G. Castillo, A. D. Paz-González, G. Rivera, M. J. Núñez, M. A. Moreno and M. L. Kennedy *
Escuela de Biología, Facultad de Ciencias Naturales y Matemáticas, Universidad de El Salvador, San Salvador, El Salvador.
09 August 2019
19 February 2021
19 May 2021
01 August 2021