ANALYSIS OF DIFFERENT BRANDS OF PARACETAMOL 500mg TABLETS USED IN HYDERABAD, USING ULTRA VIOLET SPECTROPHOTOMETRIC AND HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC (HPLC) METHODS

The study involves quantitative analysis of seven different brands (samples) of Paracetamol 500mg tablets used in Hyderabad (India), using Ultra Violet Spectrophotometric and High Performance Liquid Chromatographic methods, in which the samples were dissolved in 0.1M NaOH and distilled water and their various absorbances determined at wavelength of 257nm and in HPLC by using methanol, water (60:40) samples are prepared. The results obtained were compared with that of the standard. Percentage content and content in mg for each sample was calculated using the absorbances (in spectroscopic method) and peak areas(in chromatographic method) of the samples, to see if it is within the specified limit by official books (99%-110% according to IP). The percentage content of the analyzed samples using HPLC method ranges from 97.96-106.98%, while using UV method it ranges from 79.64-107.52%, indicating none of the samples contains less than 75% of the active principle. It was observed that four samples(B,C,D,G) out of the eight  meet up the IP specified limit in spectrophotometric method, whereas six samples(A,C,D,E,F,G) ) out of the eight  meet up the IP specified limit in chromatographic method. After the calculation of the standard deviation and coefficient of variation of the two methods used, which area108.48 and 22.58%respectively for UV method and108.04 and21.2 % respectively for HPLC method, it was also observed that the HPLC method is slightly more suitable for such kind of studies than the UV method.