ANALYSIS OF MULTIPLE ANTIBIOTICS RESISTANT AND MOLECULAR EPIDEMIOLOGY OF ESCHERICHIA COLI AND PSEUDOMONAS AERUGINOSA

The present investigation aimed at the study of resistant plasmid patterns of multidrug-resistant Escherichia coli and Pseudomonas aeruginosa isolated from hospital-acquired infection in urinary tract site are causes serious public health hazard throughout the world. A total of 100 urine samples were collected from patient admitted to the hospital. The antibiotic sensitivity of isolated uropathogens was performed by Kirby- Bauer method. The plasmid profiles of multidrug-resistant isolates were extracted by the alkaline lysis method. The multiple antibiotic-resistant indexes of uropathogens were calculated and phylogenetically determined. Among 100, 23 urine samples were positively isolated and identified. The Escherichia coli and was found to be predominantly followed by Pseudomonas aeruginosa. The multiple antibiotics resistant index was calculated as 0.75 and 0.464 respectively for Escherichia coli HAUTI8, and Pseudomonas aeruginosa HAUTI18, and these strains were phylogenetically identified. The Plasmid profile of Escherichia coli and Pseudomonas aeruginosa indicates wide ranges of plasmid among the resistant strains between 23,130 to 2027 base pairs. As a result, multidrug-resistant was an emerging disseminated clinical problem rapidly enhancing throughout the world.