ANALYSIS OF PROTEOMIC DIVERSITY AND CALCIUM BINDING PROTEIN(s) IN SEEDS OF HORSE GRAM (MACROTYLOMA UNIFLORUM) CULTIVARS FROM UTTARAKHAND

Proteomic study of Macrotyloma uniflorum is quite useful for exploration of its Calcium binding proteins (CBPs) and their antilithiasis character. CBPs play an important role in prevention of kidney stone formation. Considering this fact the study was design to identify CBPs in M. uniflorum. Total six samples were collected from different elevations of Uttarakhand. Total buffer soluble proteins of seeds were extracted according to the standard protocols. After quantification of protein with Bradford method 20 µg protein was loaded in 12% sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) gel according to Laemmli discontinuous system. CBPs were identified with the help of stains-all gel staining methods. In the proteomic study a protein (~44KDa) showed its distinct appearance when screened, the seeds from high elevation to low elevation. Three blue bands (CBPs) appear in all samples after stains-all staining. One high molecular weight protein (~43KDa) and two low molecular weight proteins (~25.1 and ~17KDa) respectively were observed. SDS-PAGE technique of seed proteins can be economically used to broad genetic variation and relatedness in cultivars and also to make distinction mutants from their parent genotype, seed protein based marker can be used for identification of genotype in future. This is a novel attempt for identification of CBPs on horse gram. CBPs have many therapeutic uses and may be used for development of antilithiatic drug formulations.