ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF PERINDOPRIL AND INDAPAMIDE USING RP-HPLC
AbstractA rapid and robust RP-HPLC method was developed and validated for the simultaneous quantification of Perindopril and Indapamide. Chromatographic separation was achieved on a Zodiac C18 column (4.6 × 150 mm, 5 µm) at 38°C using a mobile phase of acetonitrile and water (40:60 v/v) with a flow rate of 1.0 mL/min. Detection was performed at 223 nm, and the injection volume was 10 µL, with a total run time of 10 minutes. The method was validated for accuracy, precision, linearity, and reproducibility. Calibration curves demonstrated excellent linearity with correlation coefficients of 0.9991 for Perindopril and 0.9998 for Indapamide. Accuracy and precision studies showed % purity values of 99.86% for both drugs, confirming the reliability of the method. The developed RP-HPLC method is rapid, precise, and suitable for routine simultaneous estimation of Perindopril and Indapamide in pharmaceutical formulations.
Article Information
23
1561-1565
583 KB
2
English
IJPSR
Banothu Bhadru * and Dasam Bhagya Lakshmi
C. M. R. College of Pharmacy, Kandlakoya, Medchal, Hyderabad, Telangana, India.
bhadru.banothu@gmail.com
05 December 2025
21 December 2025
31 December 2025
10.13040/IJPSR.0975-8232.17(5).1561-65
01 May 2026
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