ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF SIMULTANEOUS DETERMINATION OF ATORVASTATIN CALCIUM AND AMLODIPINE BESILATE IN TABLET DOSAGE FORM BY RP-HPLCAbstract
A high performance liquid chromatography method was developed for the simultaneous separation and quantization of Atorvastatin calcium and Amlodipine besilate in combined pharmaceutical tablet dosage form. The chromatographic estimation was achieved by using mobile phase consisting of a mixture of phosphate buffer (1ml ortho phosphoric acid in 1000 ml 0f water) acetonitrile and methanol (53:43:4, v/v) at a flow rate of 1 ml/min and UV detection at 246 nm, using photodiode array detector. The determination was carried out by using Grace Smart RP C-18 column (250 X 4.6 mm, 5 µm). The retention time of Amlodipine besilate and Atorvastatin calcium was 3.337 and 6.067 respectively. Amlodipine besilate and Atorvastatin calcium calibration curves were linear with correlation coefficient 0.9989 and 0.9981 over a concentration range of 40-60 µg/ml and 80-120 µg/ml respectively. Recovery was between 99.60-100.02 for AML and 99.05-100.52% for ATV, respectively.
Antaryami Jena*, M. Madhu and S. Latha
Department of Pharmaceutical Technology, Anna University Tiruchirappalli, Tamilnadu, India
02 July, 2010
19 September, 2010
02 October, 2010
01 November, 2010