ANALYTICAL METHOD DEVELOPMENT AND VALIDATION STUDIES FOR SIMULTANEOUS ESTIMATION OF LULICONAZOLE AND EUGENOL USING UV SPECTROSCOPY
AbstractA simple, precise yet novel UV spectrophotometric method has been developed for the simultaneous estimation of luliconazole and eugenol. Two methods, namely the simultaneous equation method and the absorbance ratio method were tried for simultaneous analysis. Since the simultaneous equation method gave the best recovery; the simultaneous method was developed for the estimation of both drugs. The wavelength of interest is the wavelength maxima of both drugs; λmax of luliconazole 296 nm and λmax of eugenol 282 nm. The correlation coefficient value (r2) was found to be 0.99 for linearity studies both in methanol and phosphate buffer. Validation studies of the developed method were conducted as per ICH guidelines. The method was precise since the percent relative standard deviation was less than 2 and accurate since the percent recovery was between 99.42% and 105.16%. The developed method can be further used for simultaneous analysis of the drugs in bulk and formulation by UV spectroscopy. The development of a suitable analytical method for quantitative estimation is essential for assay and release studies.
Article Information
23
2088-2093
604 KB
277
English
IJPSR
N. Nilawar and T. Khan *
Department of Pharmaceutical Chemistry and Quality Assurance, SVKM’s Dr. Bhanuben Nanavati College of Pharmacy, University of Mumbai, Mumbai, Maharashtra, India.
tabassum.khan@bncp.ac.in
22 January 2024
24 March 2024
24 April 2024
10.13040/IJPSR.0975-8232.15(7).2088-93
01 July 2024
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