ANTI-ALBUMIN DENATURATION, TRYPSIN INHIBITION AND RBC MEMBRANE STABILIZATION AS POSSIBLE MECHANISM FOR ANTI-INFLAMMATORY ACTIVITY OF METHANOL LEAF EXTRACTS OF SOLANUM SPECIES
AbstractIn view of rising concern of problem associated with synthetic drugs against inflammation, current research has been focused towards safer, effective and natural compounds. Hence, we aimed at assessing the anti-inflammatory effect of four plants from Solanum species (S. seaforthianum, S. diphyllum, S. sisymbriifollium and S. anguivi) through albumin denaturation assay, trypsin inhibition and RBC membrane stabilization assays. The data showed that the methanol extracts of these plants had concentration dependent protection. S. anguivi extract showed maximum inhibition of albumin denaturation (26.15 ± 0.08%), whereas S. sisymbriifollium extract displayed maximum (16.51 ± 0.002%) trypsin inhibition. With respect to heat induced RBC lysis, S. anguivi extract showed highest inhibition (26.16 ± 0.08%), whereas S. seaforthianum extract displayed maximum inhibition (36.49 ± 0.002%) of RBC lysis induced by hypotonicity. From the study, it can be inferred that anti-inflammatory activity in the tested Solanum species extract varied considerably and its activity can be attributed to its richness in phytochemical compounds.
Article Information
11
726-732
2305 KB
380
English
IJPSR
M. Renu Priyadarshini and N. Lakshmi Devi *
DOS in Microbiology, University of Mysore, Manasagangotri, Mysore, Karnataka, India.
lakshmiavina@rediffmail.com
21 July 2023
29 September 2023
30 December 2023
10.13040/IJPSR.0975-8232.15(3).726-32
01 March 2024