ANTI-GLYCATION EFFECT OF WHITTON ROOT (EULOPHIA NUDA) IN-VITRO CONDITIONAbstract
Non enzymatic glycation takes place when elevated levels of reduced sugars react with amino groups of proteins and is called as advanced glycation end products (AGEs) are responsible for Diabetes Mellitus. Hydroalcoholic extract of Whitton root (Eulophia nuda) was tested for in-vitro inhibition of non-enzymatic glycation of Immunoglobin G. Plant extracts have their own importance and now being studied extensively due to having little or no side effects in all aspects of life sciences from botany to medicine in biochemical research. In present study Whitton root was selected and used to check the glycation inhibitory activity in-vitro condition. Various combinations of glucose, protein and Whitton root extracts were made under in vitro conditions and their activity was monitored with Trichloro acetic acid treatment method at 350 nm. Glycated products/ AGEs were more with high glucose and high protein concentration and these were decreased by highest concentration of Whitton root extract i.e. 30 mg/mL or 300 μL. Lower concentrations of plant extract produced either no or least response against advanced glycation end products (AGEs).
Dinesh Prasad Yadav*, Ram Charan Chhipa and Balvinder Singh
Department of Biochemistry, Suresh Gyan Vihar University, Mahal Jagatpura, Jaipur, Rajasthan, India
29 May, 2012
18 July, 2012
29 August, 2012
01 September, 2012