ANTIBACTERIAL ACTIVITIES OF OLD AND YOUNG LEAVES OF OLEA EUROPAEA (OLIVE TREE) OF ALJOUF REGION, SAUDI ARABIA

Antibacterial activities of different solvent extracts of young and old leaves has been evaluated against Pseudomonas aeureginosa (ATTCC10145) an opportunistic bacteria by Agar Well Diffusion Method. Extracts of olive leaves have been prepared by using standard extraction methods and DMS was used as vehicle for all the extracts. Amikacin was used as standard drug to which P. aeruginosa was found sensitive. Augmentin to which this bacteria is resistant was used to check combine effect (if any) with olive leave extracts. Ether, Chloroform and Alcoholic extracts of old and young leaves with or without Augmentin supplementation were used to evaluate the antibacterial activities against P. aeruginosa and all of these extracts were found active. The effect was displayed as concentration dependent for all the extracts tested in this study. The ether extract showed maximum activity which was more than the alcohol, chloroform extracts and Amikacin used as standard in this study. Augmentin supplementation to these extracts does not exert any additional inhibitory effect in the activities of olive leave extracts. Minimum inhibitory concentration was also determined and it was found that extracts of both old and young leave have inhibitory effect of which was found concentration dependent. There was no significant difference between antibacterial activities of old and young leaves. The outcome of present study suggests that these extracts have every potential to be used as neutraceuticals