ANTIBACTERIAL AND ANTIFUNGAL ACTIVITIES OF VARIOUS BREAD FRUIT LEAVES (ARTOCARPUS ALTILIS (PARKINSON) FOSBERG)

Breadfruit (Artocarpus altilis (Parkinson) Fosberg) empirically has been used in certain communities in Indonesia as a traditional medicine. Biosynthesis process can change colour leaves of breadfruit from green leaves (GL), yellow leaves (YL), fallen yellow (FY), fallen dry (FD), and the process fermentation of green leaves become fermented green (FG). The purpose of this study was to determine whether the methanol extract of breadfruit leaves variations have activity to Escherichia coli, Staphylococcus epidermidis, Propionibacterium acnes and Candida albicans and determination of Minimum Inhibitory Concentration (MIC). Simplicia was processing maseration used methanol, phytochemical screening on the fifth methanol extracts, botanicals characterization, activity test against to Escherichia coli, Staphylococcus epidermidis, Propionibacterium acnes, and activity test against to Candida albicans. Active extracts determined Minimum Inhibitory Content (MIC) and TLC. MIC used agar diffusion method. The test results showed antibacterial and antifungal activity variations of fermented green gives effective results than other breadfruit leaf variations. MIC values for the bacterium Escherichia coli, Staphylococcus epidermidis, Propionibacterium acnes, and the Fungi Candida albicans in a row by fermentated green extract 20mg/mL; 47,5mg/mL; 15mg/mL; and 475mg/mL. Test results on a TLC bioautography ethyl acetate fraction with the developer n-Hexane: Ethyl Acetate (7:3) provide clear zone at Rf 0.08; 0.13; 0.23; 0.28; 0.32; 0.38; 0.42; 0.50; 0.55; 0.60; and 0.63.