ANTIBIOTIC SUSCEPTIBILITY PATTERN OF ESBL PRODUCING BACTERIA FROM URINARY TRACT INFECTION (UTI)

Antibiotics inhibit bacterial cell wall synthesis, protein synthesis or nucleic acid replication. The antibiotic must have access to and bind to its bacterial target site. Whether antibiotic resistance is intrinsic or acquired, the genetic determinants of resistance encode specific biochemical resistance mechanism that may include enzymatic inactivation of the drug, alteration to the structure of the antibiotic target site and changes that prevent access of an adequate concentration of the antimicrobial agents to the active site. Klebsiella organisms are often resistant to multiple antibiotics. Current evidence implicates plasmids as the primary source of the resistance genes. The susceptibility pattern of different antibiotics to ESBL producing Escherichia coli and Klebsiella spp. isolated from urine samples and they found that most of the infection caused by E. coli (61.29%) found to be most common bacteria in urinary tract infection followed by Klebsiella spp. (45.16%). Isolates were highly resistance to gentamicin followed by tetracycline, ampicillin, and amikacin. gentamicin showed resistance against E. coli and Klebsiella spp were 89.47% and 85.71% respectively. While tetracyclin and ampicillin showed 80% and 78% resistance to E. coli and Klebsiella spp. The isolates were highly susceptible and least susceptible to ciprofloxacin and norfloxacin against E. coli and Klebsiella spp. These antibiotics are considered as appropriate antimicrobials for empirical treatment of urinary tract infections. Most of the ESBL A producing isolates were multidrug-resistant. During present study, when urine sample inoculated on selective media like EMB, CLED, MSA, PIA and CIA, the growth of isolates on the medium were obtained as E. coli, Klebsiella pneumonia, S. aureus, and Pseudomonas aeruginosa.