ANTIFUNGAL ACTIVITY OF TRYPSIN INHIBITORS FROM THE SEEDS OF ABELMOSCHUS MOSCHATUS
AbstractThe aim of the present study was to investigate the antifungal potential of trypsin inhibitors (AMTI-I and AMTI-II) isolated from the seeds of Abelmoschus moschatus on selected pathogenic fungal strains. The purified inhibitors have been found to be homogenous by the criteria of native PAGE and gel filtration with apparent molecular weights of 22.4kDa and 21.2 kDa as judged by SDS-PAGE. While both the inhibitors were strongly active against bovine trypsin, they showed moderate effect on porcine elastase. AMTI-I and AMTI-II significantly affected the growth of Candida albicans, Candida tropicalis, Asperigillus flavus, Saccharomyces cerevisiae, Candida glabrata and Asperigillus niger with notable zones of inhibition. The inhibitors, however, did not show any inhibitory effect on the growth of other fungal strains- Fusarium oxysporum, Alternaria alternate, Mucor indicus and Penicillium chrysogenum. The fungicides, Flucanazole and Ketoconazole were used as positive controls in this study. Results obtained suggest that AMTI-I and AMTI-II may be regarded as excellent candidates for the development of novel antimicrobial agents against human pathogenic diseases
Article Information
31
3920-27
451
1212
English
Ijpsr
Muni Kumar Dokka, Lavanya Seva and Siva Prasad Davuluri *
Department of Biochemistry, Andhra University, Visakhapatnam, Andhra Pradesh, India
sivaprasaddav@yahoo.co.in
23 February, 2015
05 June, 2015
19 June, 2015
10.13040/IJPSR.0975-8232.6(9).3920-27
01 September, 2015
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