ANTIMICROBIAL ACTIVITY OF SECONDARY METABOLITES PRODUCED BY ENDOPHYTIC FUNGI ISOLATED FROM STEMS OF JATI TREE (TECTONAGRANDIS L.F)

Diaporthephaseolorum is endophytic fungi isolated from Jatitree (Tectonagrandis L.f).  Endophytic microbes were microbes that spent part or all of their life live within plant tissue of a host plant.  They produce secondary metabolites with potent anti microbial activity.  Jatitree (Tectonagrandis L.f) was one of herbal plants that act as host plants for endophytic microbes that produce secondary metabolites with anti-microbial activity.  The current study focussed on isolation of the endophytic microbes from branches of Jati tree and their secondary metabolites.  Isolation of endophytes were performed in PDY (potato dextrose yeast) and further extracted using EtoAC.  Four fractions (F I – F IV) was obtained from EtOAc extract through column chromatography (n-Hex: EtoAc, ratio 50:1).  Further, agar diffusion method was performed to assess their anti-microbial activity.  Results demonstrated that F IV has potent towards Staphylococcus aureus (11.10mm).  Its GC-MS profile strongly indicated the presence of fatty acid and phenolic related compound.  In conclusion, secondary metabolites of endohpytes isolated from Jati tree branches contained predominantly phenol and fatty acid related compound that could be responsible for its potent anti microbial activity.