ANTIOXIDENT, ANALGESIC AND CYTOTOXIC ACTIVITY OF METHANOLIC EXTRACT OF VANDA ROXBURGHII ROOT

The crude methanolic study of Vanda roxburghii root was investigated for antioxidant, analgesic and cytotoxic activity. The extract of Vanda roxburghii root showed antioxidant activity in DPPH radical scavenging activity, nitric oxide scavenging power assay. In DPPH and NO scavenging assay the extract shown adequate antioxidant activity and the IC₅₀ values in DPPH radical scavenging and NO scavenging assays were found to be 113.35±1.27 and 127.31±0.26ug mL^-1 while the IC₅₀ values of ascorbic acid were 12.30±0.11 and 18.64±0.22 ug ml^-1. Reducing power activity of the extract increased in a dose dependent manner. Analgesic activity of the crude extract was evaluated using acetic acid induced writhing model of pain in mice. The crude extract at 200 and 400 mg kg^-1b.wt. doses displayed significant (p<0.001) reducing in acetic acid induced writhing in mice with a maximum effect of 75.89% reduction at 400 mg kg^-1b.wt. Which is comparable to the standard diclofenac sodium (86.52%). The extract was also investigated for cytotoxic activity using Brine Shrimp lethality bioassay. In this the extract showed significant toxicity of Brine Shrimp nauplii with the LC₅₀ value of 25.19+-0.98 ug mL^-1. The study indicates that the extract possesses good analgesic and cytotoxic activity along with moderate antioxidant activity.