ANXIOLYTIC AND MOTOR COORDINATION ACTIVITY OF ETHANOLIC AND AQUEOUS EXTRACTS OF DENDROPHTHOE FALCATA LEAVES IN MICEAbstract
The aim of the present study was to evaluate the anxiolytic effects and motor activity of ethanolic and aqueous extracts of leaves of Dendrophthoe falcata (DF) in mice. Swiss albino mice were treated orally with ethanolic and aqueous extracts of DF leaves (250 mg/kg and 500 mg/kg) and distilled water along with alprazolam (1 mg/kg) intraperitoneally for seven days and then exposed to Elevated Plus Maze (EPM) and Light Dark Box (LDB). The effects of the plant extracts on motor coordination were evaluated by Rota rod test. We further investigated the mechanism of action underlying the anxiolytic-like effect of the extracts by pre-treating animals with antagonist of benzodiazepine (flumazenil, 3mg/kg) prior to evaluation using EPM and LDB. Mice treated with ethanol DF extract (250 mg/kg) showed significant increase in; percentage of open arm entries, time spent in open arms of EPM and decrease in motor coordination in Rotarod test. Furthermore, the ethanol DF extracts (250 mg/kg and 500 mg/kg) significantly increased the percentage of time spent and number of transitions in the Light box in LDB test, comparable to that of the alprazolam, indicating the anxiolytic effects of the substance. The result shows that the DF extracts might dose-dependently increase chloride ion influx, which was blocked by co-administration of negative allosteric modulator, flumazenil, suggesting a GABA(A) receptor mediated mechanism of action. Taken altogether, the present study demonstrates that the ethanolic leaves extract of DF has anxiolytic activity. The extract might act at the benzodiazepine recognition site of the GABA-benzodiazepine receptor complex
Dinesh Aryal* and Nadeem Khan
Swift School of Pharmacy, Ghaggar Sarai, Rajpura, Patiala (Punjab) 140401, India.
26 August, 2014
21 November, 2014
27 December, 2014
01 April, 2015