APPLICATION OF STABILITY INDICATING UV SPECTROPHOTOMETRIC METHOD FOR ESTIMATION OF CAFFEINE IN GEL FORMULATION, PLASMA AND URINE
AbstractA simple, sensitive and stability indicating UV spectrophotometric method was developed and validated for the estimation of caffeine in a gel formulation, plasma and urine samples using acidified alcohol as solvent. At absorbance maxima of 265 nm, the linearity was found to be in range of 4-20 µg/ml with correlation coefficient 0.9988. The method was applied for estimation of caffeine in a gel formulation, plasma, and urine samples and the results show excellent recoveries. The method was validated statistically and by recovery studies for linearity, precision, repeatability, and reproducibility. The caffeine was exposed to thermal, photolytic, hydrolytic, and oxidative stress conditions, and the stressed samples were analyzed using the proposed method to demonstrate the specificity of the method. The stated method can be used as a stability-indicating method with high degree of linearity, accuracy and precision for estimation of caffeine in gel formulation and biological samples.
Article Information
31
5481-5488
855
1098
English
IJPSR
T. H. Kumar *, P. Mounika, S. S. Likhitha, S. Aswini, T. Kavya, T. Sudandhini and A. V. Sree
Vignan Institute of Pharmaceutical Technology, Duvvada, Vishakapatnam, Andhra Pradesh, India.
hemkar_pharma@yahoo.co.in
23 March 2019
06 July 2019
16 November 2019
10.13040/IJPSR.0975-8232.10(12).5481-88
01 December 2019
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