ASSESSMENT OF BIOTHERAPEUTIC POTENTIAL OF PIMENTA DIOICA (ALLSPICE) LEAF EXTRACTAbstract
All-spice (pimenta) is one of the under-utilized resources available in the tropical regions of the globe. It is a variety of sweet pepper used as a spice and its leaves are used for traditional culinary purpose. Researchers have studied the antioxidant potentials of the berries of the plant, but no documented work is reported on its stem, leaf and roots for antimicrobial properties. Thus, the present investigation was carried out to access the antimicrobial and anti-oxidation potentials of leaf extracts using three solvent systems, (Aqueous, acetone and methanol). All solvent systems at different concentrations were evaluated for antibacterial, antifungal and reducing capacity against selected bacterial and fungal pathogens; zone of inhibition was exhibited by methanol leaf extracts in decreasing order for Escherichia coli, Bacillus cereus, Salmonella typhimurium, and Staphylococcus aureus. Lesser inhibitory zones were obtained by acetone leaf extracts, whereas, Klebsiella pneumonia and Pseudomonas aeruginosa were not inhibited by any extracts. Aqueous extract demonstrated no inhibitory activity against tested bacterial pathogens. All the three leaf extracts were found to be ineffective against fungal strains (Fusarium oxysporum, Aspergillus niger, Aspergillus flavus and Candida albicans) tested. Protein content in each extract was determined and reducing capability was estimated which was found to be high in methanol and acetone extract whereas aqueous extract showed low reducing ability.
Pratima Khandelwal*, Raje Siddiraju Upendra , Zeynab Raftaniamiri, Geetha Gujjar Ramachandra
Professor & HOD, Dept. of Biotechnology, New Horizon College of Engineering, Ring Road, Bellandur Post, Marathahalli, Bangalore -560103, Karnataka, India
24 May, 2012
02 July, 2012
28 August, 2012
01 September, 2012