ASSOCIATION OF OCT1 GENE POLYMORPHISM WITH GLYCEMIC STATUS AND SERUM METFORMIN LEVELS IN TYPE II DIABETES MELLITUS PATIENTS

Aim: To investigate the possible association of OCT1 rs122083571 polymorphism (C/T allele) with glycemic status and serum metformin levels in type II Diabetes mellitus.

Methods: Sixty already diagnosed T2DM patients on metformin monotherapy (500 mg, bd) were recruited for the study and the genotypes for SLC22A1 rs122083571 C/T polymorphism using PCR assay were done. Serum fasting plasma glucose (FPG), Postprandial Plasma Glucose (PPG), Glycated Hemoglobin (HbA1c), Fasting Serum Insulin (FINS), Triacylglycerol (TG), Cholesterol (CHO), Low-Density Lipoprotein (LDL-c), High-Density Lipoprotein Cholesterol (HDL-c), and Homeostasis Model Assessment for Insulin Resistance (HOMA-IR), Body Mass Index (BMI), blood pressure (BP) were determined.

Results: In patients with C allele, the BMI,BP ,FPG ,PPG and Insulin levels were significantly  higher when compared to that of the T allele subjects .The values of TGL and HDL  in both groups of T2DM patients were not significantly different. But the total cholesterol level and LDL level were significantly increased in T2DM patients with C allele.

Conclusion: SLC22A1 rs122083571 C allele is associated with poor glycemic status in spite of     high blood metformin levels and the BMI, fasting plasma glucose, insulin ,total and LDL cholesterol levels  were also significantly higher.