BETA-GLUCOGALLIN PREVENTS SCOPOLAMINE-INDUCED AMNESIA VIA CHOLINERGIC MODULATION AND ANTI-OXIDATIVE EFFECTS IN MICE

Background: Beta-glucogallin (BGG) is a tannin precursor naturally found in a variety of plants such as gooseberry, raspberry, amla, etc. BGG was reported to be a potential therapeutic agent in managing a variety of diseases. This investigation was intended to assess whether BGG alleviates amnesia induced by scopolamine in mice. We also investigated possible mechanisms associated with the cholinergic system and antioxidant effects. Materials and Methods: Two doses of BGG (5 & 10 mg/kg) were tested in the exteroceptive and interoceptive amnesia model: Morris Water Maze test, Elevated plus-maze model, scopolamine-induced amnesia and assessment of motor coordination activity. The assessment of in vitro antioxidant activity by DPPH method and molecular docking analysis was also performed. Results: Pre-treatment with BGG at doses 5 and 10 mg/kg have shown significant anti-amnesia activity in the behavioral models in a dose-dependent manner.BGG at a lower dose did not impact the motor coordination activity of mice; however, at a higher dose, the results did not show a major impact, though the results look statistically significant compared with the control group. The results also showed that the radical scavenging activity of BGG increased with increasing concentration. Conclusion: This investigation indicates that BGG exerts potent anti-amnesic effects via cholinergic modulation and antioxidant activity, thus providing evidence for its potential as a cognitive enhancer for amnesia.