BINDING TO AND RETENTION BY MUCOSAL CELLS OF THE TAMARINDUS INDICA SEED POLYSACCHARIDE: VISUAL EVALUATION BY MEANS OF INORGANIC AND ORGANIC MARKERS
AbstractThe aim of this study was to investigate the possibility of using inorganic and organic markers to visualize the ability of the transparent polysaccharide (TSP) polymer isolated from the endosperm of the seed kernel of Tamarindus indica, a tree that mainly grows in India and South-East Asia, to bind to human mucosal cells. A layer of human buccal cells was prepared on slides and overlaid by 0.2 ml of 0.6, 0.3, 0.15 and 0.075 % TSP solutions in phosphate buffer and then colloidal carbon black particles were deposited on the slides. The unbound colloidal carbon black particles were cleared by thoroughly washing the slides. The slides were then examined by means of Nomarski interference contrast microscopy in order to visualize the degree of surface retention of the black particles by the buccal cells. The same procedure was followed using Escherichia coli as organic markers. The clearly visible binding of black carbon particles to the cells treated with polymer revealed the presence of a thin layer of TSP covering the cells (untreated cells had no black carbon particles binding). The presence of the TSP has also been confirmed by a significant reduction in bacterial adhesiveness. Both markers made it possible to visualize the binding of the thin transparent layer of TSP and its retention, which was proportional to the degree of dilution. Using Escherichia coli it has been observed the possibility of counteracting the lock-and-key mechanism of micro-organism adhesion using the bioadhesive properties of this polymer to prevent possible contact between microorganism adhesins and complementary receptors
Article Information
31
1735-1740
605KB
912
English
IJPSR
P.C. Braga*, M. Dal Sasso, M. Culici
Department of Pharmacology, School of Medicine, University of Milan, Milan, Italy
15 February, 2012
30 March, 2012
23 May, 2012
http://dx.doi.org/10.13040/IJPSR.0975-8232.3(6).1735-40
01 June, 2012