BIOANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF NITROFURANTOIN IN HUMAN PLASMA BY LC -MS / MSAbstract
A simple, rapid and sensitive method using an isocratic Liquid chromatography coupled with Tandem mass spectrometry was developed and validated for the assay of nitrofurantoin in the Human Plasma. The Mass transition of nitrofurantoin and losartan (Internal standard) were M/z 237.1/151.9 and M/Z 421.300/ 179 in ESI Negative ionization. Linearity was observed between the nitrofurantoin concentration and the peak area ratio from 10.451 to 1033.897 ng/mL with R2 value of 0.99. Plasma samples containing nitrofurantoin were extracted with Acetonitrile: 5mM Ammonium Acetate (pH 3.8) (80:20). The observed recovery of nitrofurantoin was 90%. The intra-day and inter-day accuracy was performed. Stability parameter was performed. The method will be used in the determination of the pharmacokinetic parameters of nitrofurantoin.
M. S. Kumar* and P. Shanmugapandiyan
V-102, Hivision Residency, Backside Siddh Convention, Kompally, Hyderabad, Telangana, India.
25 February, 2017
09 May, 2017
27 May, 2017
01 October, 2017