BIOMOLECULAR CHANGES DUE TO STREPTOCOCCUS MUTANS INFECTION AND ANTIBIOTICS TREATMENT IN THE HUMAN DENTAL CARIES – SILKWORM (BOMBYX MORI) DISEASE MODEL

Changes in the hemolymph protein and trehalose contents of the silkworm, Bombyx mori L. due to Streptococcus mutans infection and antibiotics treatment was evaluated. Interestingly, S. mutans, which was successfully grown in the body of the larvae led to decline 69.60 and 50.21% of protein content in the hemolymph. Concomitantly, there was a drastic rise in the content of trehalose to 60.22 and 65.13% due to bacterial infection-induced through hemocoel and oral routes respectively compared to healthy larval batches. Antibiotics clinically advocated to cure dental caries was substantially lowered the bacterial infection in the silkworm larvae regulating a rise in the protein and reduction in the trehalose contents. Notably, antibiotic-Advent treatment through hemocoel and oral routes explicit higher rate of recovery in the synthesis of protein that amounts to 133.79 and 92.62%, while trehalose content was found 34.30 and 40.31% less in the hemolymph that equivalent to healthy larvae respectively. Similarly, taxim was also influenced for an elevation of protein content 106.73 and 78.99%, whereas there was a reduction of 24.47 and 33.70% in the trehalose content of the hemolymph in the larvae received antibiotics through hemocoel and oral routes respectively. Among the two antibiotics, the efficacy of advent was much superior over taxim, despite both the antibiotics showed dose, concentration and drug dependent effects against S. mutans infection by envisaging that silkworm larvae shall be the promising human dental caries disease model to evaluate novel drugs for its toxicity and efficacy before subjecting them for clinical trials.