BIOSYNTHESIS OF SILVER NANOPARTICLES FROM HIBISCUS ROSA SINENSIS: AN APPROACH TOWARDS ANIMICROBIAL ACTIVITY ON FISH PATHOGEN AEROMONAS HYDROPHILAAbstract
Aeromonas hydrophila is a gram negative bacterium which is primary or secondary cause of ulcers and hemorrhagic septicaemia in fish. The treatments for this infection are only restricted to some antibiotics. So, novel materials are being searched for combating with bacterial infections and the resulting consequences. In this presence study, the green synthesized silver nanoparticles using Hibiscus rosa sinensis extract act as reducing agent. The silver nanoparticles have shown bactericidal effects against A. hydrophila isolated from infected fish Catla catla. The silver nanoparticles were characterized by using UV–Visible, fourier transform infra-red spectroscopy (FT-IR) and scanning electron microscopy (SEM) techniques. SEM results showed that the particles were in the size range of 5µm. FT-IR prediction indicated the presence of C–H stretch, C–H bend, N–H bend, –C≡C– stretch, H–C=O: C–H stretch, C–H stretch respectively on the AgNPs. The maximum zone of inhibition 10 mm observed by normal cured against A. hydrophila is. The synthesized silver nanoparticles exhibited maximum zone of inhibition 11 mm against A. hydrophila. As a conclusion of the study, these biologically synthesized nanoparticles were found to be highly effective against A. hydrophila infected fish. This study revealed that H. sinensis synthesized silver nanoparticles exhibit good and potent antimicrobial activity against fish pathogen.
R. Vijayaraj and N. S. Kumaran*
Department of Marine Biotechnology, AMET University, Kanathur, Chennai - 603112, Tamil Nadu, India.
16 March, 2017
02 November, 2017
11 November, 2017
01 December, 2017