CARDIOPROTECTIVE EFFECT OF FULVIC ACID ON DOXORUBICIN INDUCED CARDIAC OXIDATIVE STRESS IN RATS

The aim of study was to evaluate cardioprotective activity of fulvic acid in doxorubicin induced cardiac toxicity. In current investigation, female/male Wistar rats were divided into five groups, normal, control, fulvic acid 100 mg/kg, fulvic acid 200 mg/kg, and fulvic acid 300 mg/kg. Doxorubicin 15 mg/kg was administered in six divided doses to all except normal group for 2 weeks. Fulvic acid was administered  to respective groups once daily for total 21 days, on the last day of study, the animals were anesthetized to record ECG and BP (by cannulating carotid artery), blood was collected from carotid artery and SGOT, LDH and CK-MB were estimated, animals were sacrificed to isolate heart and preparation of tissue homogenate. The antioxidant status is analyzed by measuring MDA content, SOD, CAT, GSH activity. The tissue sample is also preserved for histological studies. Doxorubicin cause cardiac damage which was manifested by alteration in serum cardiac markers, antioxidant markers, ECG and hemodynamic and histological changes. Which were assuaged due to treatment with fulvic acid 300 mg/kg. With data obtained in study it have been concluded that fulvic acid treatment for 3 weeks shield the heart of rat from cardiotoxicity.