CHARACTERIZATION AND EVALUATION FOR ANTIBACTERIAL ACTIVITY OF GREEN SYNTHESIZED NANOPARTICLES OF LEONOTIS NEPETIFOLIA

The current research is to develop an easy and eco-friendly method for synthesizing Flower silver nanoparticles [FAgNPs] and Stem silver nanoparticles [SagNPs] using an aqueous extract of flower and stem Leonotis nepetifolia, (L.) R. Br. (family: Lamiaceae), and to evaluate their antibacterial properties. The obtained sample of synthesized FAgNPs and SAgNPs were characterized by UV–vis spectrophotometer, Zeta potential, X-ray Diffraction [XRD], Field Electron- scanning electron microscope [FE-SEM], and Fourier transform infrared spectroscopy [FT-IR] analysis. The formation of FAgNPs and SAgNPs was confirmed by the observation of band between 300 nm to 700 nm UV–vis spectrum. The crystalline structure of FAgNPs and SAgNPs with a face- centered cubic (FCC) was confirmed by XRD. The responsible phytochemicals for the reducing and capping agents of FAgNPs and SAgNPs were observed with FT-IR spectra analysis. The FE-SEM monograph revealed the spherical shape and size of FAgNPs and SAgNPs. Hence synthesized AgNPs were found to be in nano size and stable. Furthermore, the biosynthesized FAgNPs and SAgNPs indicated adequate antibacterial activity against clinical pathogen, Staphylococcus aureus [Gram-positive] and Klebsiella pneumonia [Gram-negative] bacteria. Minimum Inhibitory Concentration (MIC) was found to be 25 μg/ml.