CHARACTERIZATION OF ANDROGRAPHOLIDE IN INCLUSION COMPLEX USING BETA CYCLODEXTRIN

Andrographolide (AG) is a purified isolate of chemical synthesis resulted from sambiloto herbs (Andrographis paniculata Nees.), which has various pharmacological actions such as anti-inflammatory, hepatoprotector, antidiabetic, inhibition of replication of the HIV virus, antimalarial, anti-arthritic, anti-hyperlipidemic, anticancer, antimicrobial, immunomodulator and antiparasitic. To enhance the ability of AG to penetrate the membrane on transdermal administration, it is important to conduct the formation of inclusion complexes using beta cyclodextrin (BCD) because it has benefit such as enhancing drug release and/or permeation and stabilizing drugs in the formulation or at the absorptive site. The inclusion complexes of AG with BCD was prepared by a solvent evaporation method with mole ratio of 1:1, 1:2 and 2:1. The solids of AG inclusion complex resulted in the BCD were characterized using physical characterization of X-ray diffraction, differential scanning calorimetry, scanning electron microscope, compared to the single compound of AG, BCD, and a physical mixture of AG-BCD. Based on the results of physical characterization, the ratio that indicates the formation of inclusion complexes AG-BCD is the mole ratio of 1:2.