CHEMICAL COMPOSITION AND ANTIBACTERIAL ACTIVITY OF LEAF ESSENTIAL OIL OF EUGENIA COTINIFOLIA SSP. CODYENSIS (MUNRO EX WIGHT) ASHTON
AbstractThe study was carried out to investigate the essential oil composition and antibacterial effects of Eugenia cotinifolia ssp. codyensis leaves. The fresh leaf essential oil was extracted hydrodistillation process using Clevanger apparatus. The compounds of the essential oil were analyzed using Gas Chromatography Mass Spectrometry (GC-MS) technique identified a total of 84 numbers of chemical constituents and resulted 99.99%. The essential oil was characterized by high content of sesquiterpene compounds (79.23%) and the major constituent being Germacrene D (17.95%). The leaf oil exhibited higher antibacterial effects against gram negative bacteria compare to gram positive bacteria. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values of the oil against the organism ranged between 0.25 – 0.75 mg/ml and 0.5 – 2.5 mg/ml respectively. The essential oil exhibited maximum inhibition at 0.25 mg/ml against Proteus vulgaris and the same inhibited Bacillus subtilis and Staphylococcus aureus at 0.75 mg/ml. The result of essential oil chemical constituents and antibacterial activity showed useful for preparation of commercial products
Article Information
38
3981-85
366
1494
English
Ijpsr
R. Sarvesan , P. Eganathan *, J. Saranya and P. Sujanapal
Plant Tissue Culture and Bioprospecting Laboratory, M. S. Swaminathan Research Foundation, Taramani, Chennai, Tamilnadu, India
eganathan@gmail.com
02 February, 2015
08 April, 2015
05 June, 2015
10.13040/IJPSR.0975-8232.6(9).3981-85
01 September, 2015
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