CHITOSAN-BASED INTRATUMORAL INJECTABLE NANOPARTICLES FOR GENE DELIVERY TO PANCREATIC CANCER CELLSAbstract
One of the obstacles to effective intratumoral gene delivery lies in low transfection efficiency of non-viral vectors. Thus, in this study we evaluate the application of Diethyl methyl chitosan (DEMC) in intratumoral gene delivery of pancreatic cancer. DEMC/ pEGFP nanoparticles are prepared by polyelectrolyte complexation. After nanoparticle characterization via atomic force microscopy (AFM), in in vivo experiments, nude mice are subcutaneously injected with AsPC-1 cell line and the created tumors are used to determine the effect of DEMC on i.t. gene delivery via fluorescence microscopy, flow cytometry and immunohistochemistry. Also the relation between tumor-injection volume ratio (TIVR) and percent of the transfected tumor cells was predicted via mathematics. Relative to the control, which is injected with plasmid alone, there is a transfection increase up to 15- folds with DEMC. This delivery system involves simple preparation procedures and can be injected directly into the site, hence should be a useful approach to plasmid-based gene transfer for pancreatic cancer local therapy.
S. Safari , H. Akbari , M. Soleimani , M.H. Zarrintan , F.A. Dorkoosh , B. Larijani , M.A. Oghabian and M. Rafiee Tehrani*
Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
20 May, 2013
20 August, 2013
26 September, 2013
01 October, 2013