COMPARATIVE SCREENING AND CHARACTERIZATION OF ANTIOSTEOPOROTIC ACTIVITY OF SESBANIA GRANDIFLORA (L) POIR CRUDE LEAF EXTRACT AND ITS NANOPARTICLE FORMULATION ON SAOS-2 CELL LINES

Increasing severity of the disease osteoporosis in postmenopausal women with long term exposure to pharmacotherapy has spurred interest in the development of using food as medicine and phyto-pharmacotherapy. We examined the effect of n-hexane crude leaves extract and its nanoparticle formulation of Sesbania grandiflora in cell proliferation and bone mineralization at different concentrations in Saos-2 cell line. Osteogenic differentiation and cell viability of Saos-2 cells were quantified by monitoring alkaline phosphatase activity and MTT assay. HPLC analysis confirms the presence of bioactive compound α tocopherol. It was found that the concentrations 10 µg & 20 µg/100 µL significantly induced cell proliferation (p<0.05), and the expression of ALP (p<0.01) was not inhibited by the presence of the bioactive compound. Our data suggest that S. grandiflora nanoparticles affect the Saos-2 cell health in higher dose concentration and will be a good natural herbal medicine candidate for the treatment of osteoporosis at lower dose concentration. Further research is required to gain insight into the plant bioactive compounds and the molecular mechanism involved in their mode of action for safe and effective exploitation.