COMPARATIVE STUDY OF ANTI-AGGREGATION PROPERTY OF LEAF PROTEINS OF BRAHMI, FENUGREEK AND TRIDAX ON THE FORMATION OF LYSOZYME AMYLOID FIBRILS
AbstractLysozyme amyloid formation is studied as model in-vitro system to find out natural or synthetic inhibitors of amyloidosis. Protein aggregation and amyloid formation is the basic reason for many neurodegenerative diseases. Indigenous medicinal plants are great source of therapeutic molecules and drugs. Ethnobotanical studies have indicated many plants have neuroprotective functions. We have studied three indigenous medicinal plant namely Bacopa monnieri (BM), Trigonella foenum-graecum (TFG) and Tridax procumbens (TP) showing various pharmacological properties as inhibitors of lysozyme aggregation. We isolated leaf proteins and did the protein aggregation studies. Anti-aggregation properties of leaf extract were carried out by following assays – DTT induced lysozyme aggregation assay, Congo red binding Assay, Thiaflavin binding assay and protein stability assay by Tryptophan Fluorescence. BM leaf protein extract showed maximum inhibition followed by Methi (TFG) and then TP.
Article Information
36
5895-5901
1026 KB
220
English
IJPSR
Sayantan Sil, Sudip Kumar Nag, Labani Hazra and Srabani Karmakar *
Kingston College of Science, Barasat, Berunanpukuria, West Bengal, India.
snat14@gmail.com
30 April 2023
27 June 2023
04 July 2023
10.13040/IJPSR.0975-8232.14(12).5895-01
01 December 2023
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