COMPARATIVE STUDY OF LEVOCETIRIZINE ELIMINATION BY PRISTINE AND POTASSIUM PERMANGANATE MODIFIED ACTIVATED CHARCOAL
HTML Full TextCOMPARATIVE STUDY OF LEVOCETIRIZINE ELIMINATION BY PRISTINE AND POTASSIUM PERMANGANATE MODIFIED ACTIVATED CHARCOAL
Jasim Hamadi Hassen * 1, Yasir Mohammad Farhan 2 and Abdalkareem Hamad Ayfan 1
Department of Pharmaceutical Chemistry 1, Department of Clinical Pharmacy 2, College of Pharmacy, University of Anbar, Ramadi, Iraq.
ABSTRACT: The elimination capacity of pristine powder activated charcoal PAC and powder activated charcoal modified with potassium permanganate MPAC to remove levocetirizine drug was investigated. The activated charcoal was impregnated with KMnO4 100 mg/L in order to improve the surface properties. The influence of concentration, time and pH on the adsorption of levocetirizine drug was conducted by means of UV-Vis spectroscopy. The experiments were carried out using two lines. Line 1; adsorption of the drug on pristine PAC and line 2; adsorption on MPAC. By batch experiments, the effect of levocetirizine concentrations of 25, 50, 75, and 100 mg/L, a reaction time of 15, 30, 45 and 60 min, and pH of 2, 4, 7 and 9 were investigated. The results showed that elimination capacity of both PAC and MPAC increased with increasing the drug concentration and time of contact and decreased with decreasing the pH. The elimination efficiency of MPAC found to be greater than that of PAC.
Keywords: |
Levocetirizine, Activated charcoal, Adsorption, Potassium permanganate, Elimination
INTRODUCTION: Activated charcoal is one of the main effective adsorbents which has a high surface area, large pore volume, tunable pore size, chemical stability and high hydrophobicity 1, 2, 3. It is in the shape of a fine black odorless, tasteless, microcrystalline powder 4. Due to its high adsorptive capacity is used as an adsorbent for a large variety of compounds. It is used for removal of colors from solutions 5, 6. Activated charcoal is used in the adsorption of gases and vapors such as ammonia, carbon tetrachloride, methane, nitrogen dioxide and carbon dioxide 7, 8, 9. A large number of studies concern the adsorption of a verity of organic compounds on activated charcoal have been conducted.
From these studies adsorption of phenol, diethyl phthalate, dibutyl phthalate and many other compounds 10, 11, 12. In the pharmaceutical field, activated charcoal has been used as an antidote for the majority of poisons because of its ability to prevent the absorption of many toxic agents and enhance the removal of many absorbed drugs. It is used as an antiflatulent, and as a good treatment to reduce blood lipid concentrations in patients having uremia and diabetes 13, adsorption of odors from wounds 14.
Activated charcoal has been used as an adsorbent for a wide range of different drugs like paracetamol 15, tramadol hydrochloride 16, diazepam 17, acetaminophen 18 and many others. In recent years, many researchers have dedicated to research on modification of activated charcoal to improve the adsorption capacity 19, 20, 21, 22. One of the important methods is to oxidize the structure by oxidizing agents 23, 24. Oxidization with ozone 25, concentrated nitric acid 26, 27 and hydrogen peroxide 28 can enhance the adsorption of functional groups of carboxylic acids and some other organic compounds. It was found that oxidizing the structure of activated charcoal change its inert and hydrophobic nature and enhance the wettability for polar solvents 29.
Granular activated charcoal modified with potassium permanganate was used to enhance the adsorption capacity for phenol. It was found that modification experiment increases the efficiency of activated charcoal adsorption by 20% 30. Modification with potassium permanganate increases the removal efficiency of activated charcoal for removal of Cr(VI) 31, 32 and Cu2+ 33 from aqueous solution. The effect of oxidation of the organic matter by potassium permanganate before adsorption on activated charcoal and oxidizing activated charcoal and then adsorbing organic matter was also investigated 34. Some researchers have found that permanganate is not adsorbed on the activated charcoal but reduced. The reduction process was found to be strongly pH dependent, and the product of reduction is mainly Mn2+. The Mn2+ ion adsorbed onto the activated charcoal particles by surface complexation 35. A study of adsorption of levofloxacin on granular activated carbon produced from date (Phoenix dactylifera L.) stones showed adsorption capacity of 100.3 mg/g 36.
The removal of levofloxacin by means of alumina-doped coconut coir charcoal impregnated with alumina nanoparticles showed that the adsorption isotherm data were best represented by both Langmuir and Temkin isotherm 37. Activated charcoal produced from sugarcane bagasse removes 37% from levofloxacin drug in 90 min reaction time. The reaction isotherm follows Langmuir model 38. The adsorption of levofloxacin drug on goethite has been studied. The study concern the effects of nitrate, sulfate, small organic acids and humic acid on the adsorption process. It was shown that the presence of nitrate or sulfate had no effect, but the presence of humic acid increase the process 39. The adsorption of levofloxacin on activated charcoal produced from rice husk was found to be spontaneous and endothermic while the adsorption on activated charcoal produced from a wood chip is spontaneous and exothermic 40. A waste produced from corn bracts and later modified using zirconium cations were used for removal levofloxacin drug from waste water. The waste exhibited 73 mg/g adsorption capacity 41. The adsorption of the gliclazide drug on activated charcoal was found to be pH, concentration and time-dependent 42. The aim of this research is to compare the elimination levels of levocetirizine by pristine and modified activated charcoal and to study the effects of concentration, time and pH on the process.
MATERIALS AND METHODS: The activated charcoal used was obtained from Sigma-Aldrich, after repeatedly washed with distilled water and dried to constant weight at 105 ºC for over 3 h. The drug sample of levocetirizine is a production of TDA Pharma. All chemicals used in this work were of analytical grade obtained from Sigma-Aldrich.
The electronic spectra were recorded on PG instruments T80 UV/Vis double beam spectro-photometer. The pH was monitored using a Philips (PW-9409) digital pH meter.
The wavelength at which maximum absorbance occurs (λmax) was recorded for the aqueous solutions of drug and found to be 230 nm.
An exact weight of 50 mg of levocetirizine was dissolved in 250 ml distilled water in a 500 ml volumetric flask. The solution was shaken to dissolve all the drug partials and the volume was adjusted up to the mark of the flask with distilled water to obtain a stock solution of 100 mg/L. The solution was filtered using Whatman filter paper no. 41.
Modification Method of Activated Charcoal: Modification of activated charcoal was conducted by immersing the powder in a solution of potassium permanganate 100 mg/L under a neutral condition for 4 h with slow stirring. The mixture then separated and washed repeatedly with distilled water to no color and dried to constant weight at 105 ºC for 4 h in order to remove the content of impregnation solvent and water.
Adsorption Studies: Adsorption experiments were performed to study the effects of important parameters such as concentration, time and pH. The experiments were performed on both pristine PAC and MPAC separately.
The flask containing 50 ml of levocetirizine solution of concentrations 25, 50, 75 and 100 mg/L were shaken with 0.5 gm activated charcoal for 1 hour for concentration study.
For time study, 50 ml of drug solution 100 mg/L were shaken with 0.5 gm activated charcoal for 15, 30, 45 and 60 min.
For pH study, 50 ml of drug solution 100 mg/L were shaken with 0.5 gram of activated charcoal for 1 h at pH of 2, 4, 7 and 9. The mixtures were shaken vigorously at room temperature on magnetic stirrer after which filtered through filter paper and the filtrate solutions were analyzed spectrophotometrically at λ = 230 for residual drug concentration. The process was repeated for PAC and MPAC under different equilibrium conditions of concentration, time and pH. The pH was adjusted using 0.1 N solutions of HCl and NaOH.
The amount of the drug adsorbed was calculated using the equation:
Qe = (Co-Ce) V / m
Where Qe is the maximum quantity of the drug in mg/gm adsorbed on the activated charcoal, Co is the initial concentration (mg/L) of the drug solution, Ce is the concentration of the drug (mg/L) in the supernatant at the equilibrium stage, V is the volume of the drug solution in liter and m is the mass of adsorbent employed in grams.
The calibration curve was conducted by measuring the absorbance of solutions for each experiment, then plotting the absorbance versus concentrations, time and pH.
RESULTS AND DISCUSSION: Levocetirizine is a selective H1-antihistamine drug used for the treatment of allergic rhinitis and chronic idiopathic urticaria. It is an off-white crystalline powder soluble in water. The chemical formula is C21H25ClN2O3. It is the R-enantiomer of racemic cetirizine. The chemical name of the drug is 2-[2-[4-[(R)-(4-chlorophenyl)-phenyl methyl] pipera-zinyl-1-yl]ethoxy] acetic acid 43, 44.
The chemical structure of the drug is shown in Fig. 1. The UV spectrum of the levocetirizine solution is shown in Fig. 2.
FIG. 1: THE CHEMICAL STRUCTURE OF LEVOCETIRIZINE
The results of concentration for both PAC and MPAC study are listed in Table 1.
FIG. 2: THE UV SPECTRUM OF LEVOCETIRIZINE
TABLE 1: PARAMETERS OF CONCENTRATION STUDY
Concentration (mg/L) | Levocetirizine Adsorbed on PAC (mg/g) | Levocetirizine Adsorbed on MPAC (mg/g) |
25 | 1.1 | 1.8 |
50 | 2.2 | 2.8 |
75 | 3.2 | 3.8 |
100 | 4.7 | 4.9 |
To simulate the adsorption process in the environment of the gastric fluid a sample was used with its additives.
It is clear from Table 1 that the amounts of the adsorbed drug in mg for each gm of both PAC and MPAC increases almost steadily with increased concentration of the drug solution. At the same time, it seems clear that MPAC adsorbs larger amounts than PAC. The amounts of 4.7 and 4.9 mg of the levocetirizine adsorbed on 1 gm PAC and MPAC respectively in the 100 mg/L concentration seem reasonable.
Fig. 3 is a plot of the amounts of the drug adsorbed on activated charcoal in mg/gm versus concentration for concentration study. The steady increscent in the amount of the levocetirizine drug adsorption on PAC and MPAC is very obvious from the plot.
The parameters for time study are listed in Table 2. When the time duration of the reaction was changed from 15, 30, 45 to 60 min, the amounts of the drug adsorbed increased with increasing the time for both PAC and MPAC. This time incensement gives the indication that the time of contact is also an important factor for the elimination of the drug. Again the adsorption rate of MPAC was greater that of PAC. The enhancement of adsorption when MPAC is used is very obvious. The relationship between time and the amounts of the adsorbed drug is shown in Fig 4. The steady increscent of the adsorption with increasing time is clear.
TABLE 2: PARAMETERS OF TIME STUDY
Time (min) | Levocetirizine Adsorbed on PAC (mg/gm) | Levocetirizine Adsorbed on MPAC (mg/gm) |
15 | 3 | 4 |
30 | 3.2 | 4.3 |
45 | 3.9 | 5 |
60 | 4.2 | 5.4 |
Table 3 contains the parameters for pH study. The amounts of the levocetirizine in mg adsorbed on 1gm activated charcoal increasing as the pH of the solution decreased. The highest values of 5.4 and 6.6 mg/gm for the PAC and MPAC respectively found at pH 2, which seems a good result since the pH of the gastric fluid in this rang. The relationship between the pH and the amounts of the adsorbed drug gave a straight line as shown in Fig 5. Only the value at pH 7 for the PAC showed deviation from the straight line.
TABLE 3: PARAMETERS OF pH STUDY
pH | Levocetirizine Adsorbed on PAC (mg/gm) | Levocetirizine Adsorbed on MPAC (mg/gm) |
2 | 5.4 | 6.6 |
4 | 4.3 | 5.8 |
7 | 4 | 4.5 |
9 | 2.5 | 3.3 |
From the parameters of the three factors, it is quite clear that the elimination of the levocetirizine drug from the solutions depends on concentration, time and pH. Modification of the activated charcoal by potassium permanganate enhance its elimination capacity in all the experiments factors used.
The enhancement capacity reaches 63% for the 25% concentration as is evident from Table 4. A quick glance at the table reveals that the MPAC adsorption capacity was enhanced by about 30% in general.
TABLE 4: % OF ADSORPTION CAPACITY ENHANCEMENT IN MPAC
% Adsorption Enhancement in concentration study | 25 | 50 | 75 | 100 |
63% | 27% | 18% | 4% | |
% Adsorption Enhancement in Time Study | 15 | 30 | 45 | 60 |
33% | 34% | 28% | 28% | |
% Adsorption Enhancement in pH Study | 2 | 4 | 7 | 9 |
22% | 34% | 12% | 32% |
FIG. 3: PLOT OF ADSORBED DRUG (mg/gm) VERSUS CONCENTRATION FOR CONCENTRATION STUDY
CONCLUSION: As a comparative investigation, powder activated charcoal PAC and powder activated charcoal impregnated with potassium permanganate MPAC were used to eliminate the levocetirizine drug from solutions. The process include different concentrations (25, 50, 75 and 100 mg/L), different times (15, 30, 45, and 60 min) and different pH (2, 4, 7, and 9). It was found that the elimination rate depends on the concentration, time and pH. The greater the concentration and time the greater the elimination capacity, and the lower the pH the greater the elimination capacity. The enhancement in elimination capacity of MPAC by about 30% was evident in all the variable factors of the experiments.
ACKNOWLEDGEMENT: Authors acknowledge Department of Pharmaceutical Chemistry and the Department of Clinical Pharmacy, College of Pharmacy, University of Anbar, Iraq for providing facilities to carry out this research.
CONFLICT OF INTEREST: The authors do not have any conflict of interest.
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How to cite this article:
Hassen JH, Farhan YM and Ayfan AH: Comparative study of levocetirizine elimination by pristine and potassium permanganate modified activated charcoal. Int J Pharm Sci & Res 2018; 9(12): 5155-60. doi: 10.13040/IJPSR.0975-8232.9(12).5155-60.
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Article Information
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967
English
IJPSR
J. H. Hassen *, Y. M. Farhan and A. H. Ayfan
Department of Pharmaceutical Chemistry, College of Pharmacy, University of Anbar, Ramadi, Iraq.
Jasimhu@hotmail.com
29 March, 2018
25 June, 2018
02 July, 2018
10.13040/IJPSR.0975-8232.9(12).5155-60
01 December, 2018