CRUCIAL ROLE OF NITROGEN IN IN- VITRO REGENERATION OF PHYLLANTHUS AMARUS SCHUM. AND THONN.Abstract
Nitrogen plays a pivotal role in growth and differentiation of cells of Phyllanthus amarus Schum. and Thonn. Both form and amount of nitrogen in nutrient medium have significant effects on the rate of cell growth and differentiation. In this study, effects of different inorganic nutrients (KNO3 and NH4NO3) and organic nutrients (glycine) on regeneration of P. amarus were evaluated. Murashige and Skoog’s medium (MS) was used as basal medium along with plant growth hormones and different concentrations of nitrogen. Among the various growth hormones tried, BAP (0.5 mg/l) gave maximum number of shoots (17.33±0.86) after 3-4 weeks of inoculation. However, various strength of KNO3, NH4NO3 and glycine were optimized in the medium in order to get the maximum number of shoots, nodes per shoot, shoot length as well as the size of leaves respectively. Amongst all modifications, less or more, MS medium with standard amount of nitrogen source was found to be most effective. The shoots regenerated on MS medium along with BAP (0.5 mg/l) were subjected to their elongation on the same medium and their root induction was done on half strength MS medium along with IBA (0.5 mg/l). These complete plantlets were hardened and acclimatized in natural conditions. 80% survival rate was observed under field conditions.
Antara Sen* and Amla Batra
Plant Biotechnology Laboratory No.- 5, Department of Botany, University of Rajasthan, Jaipur, Rajasthan, India
27 April, 2011
07 June, 2011
12 July, 2011
01 August, 2011