DESIGN, DEVELOPMENT AND STATISTICAL OPTIMIZATION OF ACYCLOVIR-LOADED PROTEIN NANOPARTICLES
AbstractTo solve the problem of low drug availability in the body, acyclovir was fabricated into biodegradable gelatin nanoparticles by step-wise two-times desolvation method where gelatin was used as biodegradable polymer and glutaraldehyde was utilized as a cross-linking agent. Optimization was conceded by design expert computational application whereby the outcome of gelatin polymer concentration (X1) and glutaraldehyde-crosslinking agent (X2) were studied on particle size (Y1), zeta potential (Y2), and entrapment efficiency (Y3). The drug-loaded gelatin nanoparticle formulations were characterized by particle size, surface charge, and entrapment efficiency. ANOVA studies also evaluated drug-loaded gelatin nanoparticles. The optimized formulation (F9) of 0.8% gelatin polymer (X1) and 250 μl of glutaraldehyde-crosslinking agent (X2) containing acyclovir: gelatin ratio of 1:8, which showed a particle size, zeta potential and maximum entrapment efficiency of 139.87 nm, -32.67mv and 91.23% respectively.
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