DETECTION OF BIOFILM PRODUCING BACTERIA ON INVASIVE MEDICAL DEVICES AND THEIR ANTIBIOGRAM

Biofilm formation is prevailing in almost all bacteria. They are responsible to cause infections like Peritonitis, Cystic fibrosis, Endocarditis, Otitis media, UTIs. The infections caused by the biofilms forming bacteria that colonize the indwelling medical devices are a major source of patient morbidity and implant failure.  The study on biofilm formation on indwelling medical devices is important from a public health perspective. In the present study we have isolated various organisms on indwelling medical devices, and tested them for biofilm formation and also antibiotic susceptibility. In the present study a total of 100 isolates are identified by standard microbiological procedures, evaluated for biofilm production from various indwelling medical devices by two different methods, Tube method and by using Congo red agar. The Results 48% of isolates showed biofilms production, Klebsiella being the predominant isolate 20 (42%). Gram negative isolates were sensitive to Amikacin and Nitrofurantoin 22 (46%).  Gram positive isolates were sensitive to Vancomycin and tetracycline 11 (23%). Congo red agar method was considered to be superior to test tube method 32 (66%), Z=3.12, P=0.002 which is considered to be significant statistically. The Biofilm producing bacteria are more in invasive medical devices and are resistant to many commonly used antibiotics. So it is an alarm for those who are associated with invasive procedures and indwelling medical devices.