DETERMINATION OF DRUG SUSCEPTIBILITY PATTERN AGAINST NOSOCOMIAL INFECTION

Nosocomial infections even in this modern era antibiotics, continue to remain an important and formidable consequence of hospitalization. Our aim of the study was to investigate the antimicrobial susceptibility against hospital-associated infecting microorganisms. 30 Clinical samples were taken from OPD of GMC Hospital, Bhopal (MP), India. Among 30 clinical isolates we identified bacterial pathogens as Staphylococcus aureus (10), Escherichia coli (10) and Pseudomonas aeruginosa (8). Antimicrobial susceptibility assay was performed by the reference criteria of clinical and laboratory standard institute guidelines. In the present study antibiotic susceptibility results showed that all 10 (100%) S. aureus isolates were resistant to penicillin, 8 (80%) S. aureus isolates were resistant to cefuroxime, 7 (70%) S .aureus isolates were resistant to ciprofloxacin, 5 (50%) S. aureus isolates were resistant to amikacin, 2 (20%) S. aureus isolates were resistant to nitrofurantoin whereas, 0 (0%) S. aureus isolates were found to be resistant to cefazolin. For E. coli isolates all 10 (100%) were resistant to cefuroxime, 7 (70%) E. coli isolates were resistant to amikacin, 5 (50%) E. coli isolates were resistant to penicillin, 2 (20%) E. coli isolates were resistant to cefazolin as well as nitrofurantoin whereas, 0 (0%) E. coli isolates found to be resistant to ciprofloxacin. For P. aeruginosa isolates all 8 (100%) were resistant to nitrofurantoin, 6 (75%) P. aeruginosa isolates were resistant to cefuroxime, 5 (62.5%) P. aeruginosa isolates were resistant to amikacin, 4 (50%) P. aeruginosa isolates were resistant to penicillin, 3 (37.5%) P. aeruginosa isolates were resistant to ciprofloxacin whereas, 2 (25%) P. aeruginosa isolates were resistant to cefazolin. Hence we concluded that the efficacy of cefazolin and ciprofloxacin was higher than other antibiotics tested against Nosocomial infection causing pathogens