DETERMINATION OF IBUPROFEN IN HUMAN PLASMA WITH MINIMAL SAMPLE PRETREATMENT
AbstractA simple, sensitive and selective high performance liquid chromatography (HPLC) method with ultraviolet detection (220 nm) was developed and validated for the quantification of Ibuprofen and in human plasma. Following rapid sample preparation, the analytes and internal standard (Diclofenac sodium) were separated using an isocratic mobile phase on a reverse phase C8 column. The lower limit of quantification was 1µg/mL for Ibuprofen. A linear dynamic range of 1µg/mL to 100µg/mL for Ibuprofen was established. This HPLC method was validated with between and within-batch precision of 1.2- 4.7% and 1.8- 4.8 %, respectively. The between and within batch accuracy was 99.4- 104.2% and 99.7- 104.1%, respectively. Frequently co-administered drugs did not interfere with the described methodology. Stability of Ibuprofen in plasma was >90%, with no evidence of degradation during sample processing (autosampler) and 60 days storage in a freezer. This validated method is sensitive and simple with between-batch precision of < 5 % and was used in pharmacokinetic studies.
Article Information
14
120-127
389 kB
1190
English
IJPSR
M. Ganesan, K. S. Rauthan, Y. Pandey and P. Tripathi*
Micro Therapeutic Research Labs Pvt. Ltd., Chennai (TN) India
prashant@microtheraps.com
02 February, 2010
15 April, 2010
22 April, 2010
http://dx.doi.org/10.13040/IJPSR.0975-8232.1(5).120-27
01 May, 2010
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